= Brainstorm-FieldTrip auditory tutorial =
==== [TUTORIAL UNDER DEVELOPMENT: NOT READY FOR PUBLIC USE] ====
''Authors: Francois Tadel, Elizabeth Bock.''

The aim of this tutorial is to provide high-quality recordings of a simple auditory stimulation and illustrate the best analysis paths possible with Brainstorm and !FieldTrip. This page presents the workflow in the Brainstorm environment, the equivalent documentation for the !FieldTrip environment will be available on the [[http://fieldtrip.fcdonders.nl/|FieldTrip website]].

Note that the operations used here are not detailed, the goal of this tutorial is not to teach Brainstorm to a new inexperienced user. For in depth explanations of the interface and the theory, please refer to the 12+3 introduction tutorials.

<<TableOfContents(3,2)>>

== License ==
This tutorial  dataset (MEG and MRI data) remains a property of the MEG Lab, !McConnell  Brain Imaging Center, Montreal Neurological Institute, !McGill  University, Canada. Its use and transfer outside the Brainstorm  tutorial, e.g. for research purposes, is prohibited without written  consent from the MEG Lab.

If  you reference this dataset in your publications, please aknowledge its  authors (Elizabeth Bock, Peter Donhauser, Francois Tadel and Sylvain  Baillet) and cite Brainstorm as indicated on the [[CiteBrainstorm|website]]. For questions, please contact us through the forum.

== Presentation of the experiment ==
==== Experiment ====
 * One subject, two acquisition runs of 6 minutes each
 * Subject stimulated binaurally with intra-aural earphones (air tubes+transducers)
 * Each run contains:
  * 200 regular beeps (440Hz)
  * 40 easy deviant beeps (554.4Hz, 4 semitones higher)
 * Random inter-stimulus interval: between 0.7s and 1.7s seconds, uniformly distributed
 * The subject presses a button when detecting a deviant
 * Auditory stimuli generated with the Matlab Psychophysics toolbox

==== MEG acquisition ====
 * Acquisition at '''2400Hz''', with a '''CTF 275''' system at the MNI, subject in seating position
 * Online 600Hz low-pass filter, files saved with the 3rd order gradient
 * Recorded channels (340):
  * 1 Stim channel indicating the presentation times of the audio stimuli: UDIO001 (#1)
  * 1 Audio signal sent to the subject: UADC001 (#316)
  * 1 Response channel recordings the finger taps in resonse to the deviants: UPPT001 (#2)
  * 26 MEG reference sensors (#5-#30)
  * 274 MEG axial gradiometers (#31-#304)
  * 2 EEG electrodes: Cz, Pz (#305 and #306)
  * 1 ECG bipolar (#307)
  * 2 EOG bipolar (vertical #308, horizontal #309)
  * 12 Head tracking channels: Nasion XYZ, Left XYZ, Right XYZ, Error N/L/R (#317-#328)
  * 20 Unused channels (#3, #4, #310-#315, #329-340)
 * 3 datasets:
  * '''S01_AEF_20131218_01.ds''': Run #1, 360s, 200 standard + 40 deviants
  * '''S01_AEF_20131218_02.ds''':  Run #2, 360s, 200 standard + 40 deviants
  * '''S01_Noise_20131218_01.ds''': Empty room recordings, 30s long
  * File name: S01=Subject01, AEF=Auditory evoked field, 20131218=date(Dec 18 2013), 01=run
 * Use of the .ds, not the AUX (standard at the MNI) because they are easier to manipulate in !FieldTrip

==== Stimulation delays ====
 * '''Delay #1''': Between the stim markers (channel UDIO001) and the moment where the sound card plays the sound (channel UADC001). This is mostly due to the software running on the computer (stimulation software, operating system, sound card drivers, sound card electronics). The delay can be measured from the recorded files by comparing the triggers in the two channels:<<BR>>Delay''' between 11.5ms and 12.8ms''' (std = 0.3ms)<<BR>>This delay is '''not constant''', we will need to correct for it.

 * '''Delay #2''': Between when the sound card plays the sound and when the subject receives the sound in the ears. This is the time it takes for the transducer to convert the analog audio signal into a sound, plus the time it takes to the sound to travel through the air tubes from the transducer to the subject's ears. This delay cannot be estimated from the recorded signals: before the acquisition, we placed a sound meter at the extremity of the tubes to record when the sound is delivered.<<BR>>Delay '''between 4.8ms and 5.0ms''' (std = 0.08ms).<<BR>>At a sampling rate of 2400Hz, this delay can be considered '''constant''', we will not compensate for it.

 * '''Delay #3''': The CTF MEG systems have a constant delay of '''4 samples'''   between the MEG/EEG channels and the analog channels (such as the  audio signal UADC001), because of an anti-aliasing filtered that is  applied to the first and not the second.<<BR>>This translate here to a '''constant delay''' of '''1.7ms'''.

 * '''Delay #4''': When correcting of delay #1, the process we use to detect the beginning of the triggers on the audio signal (UADC001) sets the trigger in the middle of the ramp between silence and the beep. We "over-compensate" the delay #1 by 1.7ms.<<BR>>This can be considered as '''constant delay '''of about '''-1.7ms'''.

 * '''Uncorrected delays''': We will correct for the delay #1, and keep the other delays (#2, #3 and #4). After we compensate for delay #1 our MEG signals will have a '''constant delay '''of about 4.9 + 1.7 - 1.7 = '''4.9 ms'''. We decide not to compensate for those delays because they do not introduce any jitter in the responses and they are not going to change anything in the interpretation of the data.

==== Head shape and fiducial points ====
 * 3D digitization using a Polhemus Fastrak device driven by Brainstorm ('''S01_20131218_*.pos''')<<BR>>More information: [[Tutorials/TutDigitize|Digitize EEG electrodes and head shape]]
 * The output file is copied to each .ds folder and contains the following entries:
  * The position of the center of CTF coils
  * The position of the anatomical references we use in Brainstorm: <<BR>>Nasion and connections tragus/helix, as illustrated [[http://neuroimage.usc.edu/brainstorm/CoordinateSystems#Pre-auricular_points_.28LPA.2C_RPA.29|here]].
 * Around 150 head points distributed on the hard parts of the head (no soft tissues)

==== Subject anatomy ====
 * Subject with 1.5T MRI
 * Marker on the left cheek
 * Processed with !FreeSurfer 5.3

== Download and installation ==
 * '''Requirements''': You have  already followed all the basic tutorials and you have a working copy of  Brainstorm installed on your computer.
 * Go to the [[http://neuroimage.usc.edu/brainstorm3_register/download.php|Download]] page of this website, and download the file: '''sample_auditory.zip '''
 * Unzip   it in a folder that is not in any of the Brainstorm folders (program   folder or database folder). This is really important that you always   keep your original data files in a separate folder: the program folder   can be deleted when updating the software, and the contents of the   database folder is supposed to be manipulated only by the program   itself.
 * Start Brainstorm (Matlab scripts or stand-alone version)
 * Select the menu File > Create new protocol. Name it "'''!TutorialAuditory'''" and select the options:
  * "'''No, use individual anatomy'''",
  * "'''No, use one channel file per condition'''".

== Import the anatomy ==
 * Switch to the "anatomy" view.
 * Right-click on the !TutorialAuditory folder > New subject > '''Subject01'''
  * Leave the default options you set for the protocol
 * Right-click on the subject node > Import anatomy folder:
  * Set the file format: "!FreeSurfer folder"
  * Select the folder: '''sample_auditory/anatomy'''
  * Number of vertices of the cortex surface: 15000 (default value)
 * Set the 6 required fiducial points (indicated in MRI coordinates):
  * NAS: x=127, y=213, z=139
  * LPA: x=52, y=113, z=96
  * RPA: x=202, y=113, z=91
  * AC: x=127, y=119, z=149
  * PC: x=128, y=93, z=141
  * IH: x=131, y=114, z=206 (anywhere on the midsagittal plane)
 * At  the end of the process, make sure that the file "cortex_15000V" is  selected (downsampled pial surface, that will be used for the source  estimation). If it is not, double-click on it to select it as the  default cortex surface.
 {{attachment:anatomy.gif||height="264",width="335"}}

== Access the recordings ==
=== Link the recordings ===
 * Switch to the "functional data" view.
 * Right-click on the subject folder > Review raw file
  * Select the file format: "'''MEG/EEG: CTF (*.ds...)'''"
  * Select all the .ds folders in: '''sample_auditory/data''' {{attachment:raw1.gif||height="156",width="423"}}
 * Refine registration now? '''YES'''<<BR>><<BR>> {{attachment:raw2.gif||height="224",width="353"}}

=== Multiple runs and head position ===
 * The two AEF runs 01 and 02 were acquired successively, the position of the subject's head in the MEG helmet was estimated twice, once at the beginning of each run. The subject might have moved between the two runs. To evaluate visually the displacement between the two runs, select at the same time all the channel files you want to compare (the ones for run 01 and 02), right-click > Display sensors > MEG.<<BR>><<BR>> {{attachment:raw3.gif||height="220",width="441"}}
 * Typically, we would like to group the trials coming from multiple runs by experimental conditions. However, because of the subject's movements between runs, it's not possible to directly compare the sensor values between runs because they probably do not capture the brain activity coming from the same regions of the brain.
 * You have three options if you consider grouping information from multiple runs:
  * '''Method 1''': Process all the runs separately and average between runs at the source level: The more accurate option, but requires a lot more work, computation time and storage.
  * '''Method 2''': Ignore movements between runs: This can be acceptable for commodity if the displacements are really minimal, less accurate but much faster to process and easier to manipulate.
  * '''Method 3''': Co-register properly the runs using the process Standardize > Co-register MEG runs: Can be a good option for displacements under 2cm. Warning: This method has not be been fully evaluated on our side, to use at your own risk.
 * In this tutorial, we will illustrate only method 1: runs are not co-registered.

=== Epoched vs. continuous ===
 * The CTF MEG system can save two types of files: epoched (.ds) or continuous (_AUX.ds).
 * Here we have an intermediate storage type: continuous recordings saved in an "epoched" file. The file is saved as small blocks of recordings of a constant time length (1 second in this case). All those time blocks are contiguous, there is no gap between them.
 * Brainstorm can consider this file either as a continuous or an epoched file. By default it imports the regular .ds folders as epoched, but we can change this manually, to process it as a continuous file.
 * Double-click on the "Link to raw file" for run 01 to view the MEG recordings. You can navigate in the file by blocks of 1s, and switch between blocks using the "Epoch" box in the Record tab. The events listed are relative to the current epoch.<<BR>><<BR>> {{attachment:raw4.gif||height="206",width="575"}}
 * Right-click on the "Link to raw file" for run 01 > '''Switch epoched/continuous'''
 * Double-click on the "Link to raw file" again. Now you can navigate in the file without interruptions. The box "Epoch" is disabled and all the events in the file are displayed at once.<<BR>><<BR>> {{attachment:raw5.gif||height="209",width="576"}}
 * Repeat this operation twice to convert all the files to a continuous mode.
  * '''Run 02''' > Switch epoched/continuous
  * '''Noise''' > Switch epoched/continuous

== Stimulation triggers delay ==
=== Evaluation ===
 * Right-click on Run01/Link to raw file > '''Stim '''> Display time series (stimuls channel, UDIO001)<<BR>>Right-click on Run01/Link to raw file > '''ADC V''' > Display time series (audio signal generated, UADC001)

 * In the Record tab, set the duration of display window to '''0.200s'''.<<BR>>Jump to the third event in the "standard" category.

 * We can observe that there is a delay of about '''13ms''' between the time where the stimulus trigger is generated by the stimulation computer and the moment where the sound is actually played by the sound card of the stimulation computer ('''delay #1'''). This is matching the documentation of the experiment in the first section of this tutorial. <<BR>><<BR>> {{attachment:stim1.gif||height="282",width="590"}}

=== Correction ===
 * '''Delay #1''': We can detect the triggers from the analog audio signal (ADC V/UADC001) rather than using the events already detected  by the CTF software from the stim channel (Stim/UDIO001).
 * Drag and drop '''Run01 '''and '''Run02 '''to the Process1 box.
 * Add __'''twice'''__ the process "'''Events > Detect analog triggers'''".<<BR>>Once with event name="standard_fix" and reference event="standard".<<BR>>Once with event name="deviant_fix" and reference event="deviant".<<BR>>Set the other options as illustrated below:<<BR>><<BR>> {{attachment:stim2.gif}}
 * Open Run01 (channel ADC V) to evaluate the correction that was performed by this process. If you look at the third trigger in the "standard" category, you can measure a 14.6ms delay between the original event "standard" and the new event "standard_fix".<<BR>><<BR>> {{attachment:stim3.gif||height="151",width="570"}}
 * Open '''Run01''' to re-organize the event categories:
  * '''Delete '''the unused event categories: '''standard''', '''deviant'''.
  * '''Rename '''standard_fix and deviant_fix to '''standard''' and '''deviant'''.
 * Open '''Run02''' and do the same cleaning operations:<<BR>><<BR>> {{attachment:stim5.gif||height="149",width="502"}}
 * '''Important note''': We compensated for the jittered delays (delay #1), but not for the other ones (delays #2, #3 and #4). There is still a''' constant 5ms delay''' between the stimulus triggers ("standard" and "deviant") and the time where the sound actually reaches the subject's ears.

== Detect and remove artifacts ==
=== Spectral evaluation ===
 * One of the typical pre-processing steps consist in getting rid of the contamination due to the power lines (50 Hz or 60Hz). Let's start with the spectral evaluation of this file.
 * Drag '''ALL''' the "Link to raw file" to the Process1 box, or easier, just drag the node "Subject01", it will select recursively all the files in it.
 * Run the process "'''Frequency > Power spectrum density (Welch)'''":
  * Time window: '''[All file]''', Window length: '''4s''', Overlap: '''50%''', Sensor types: '''MEG'''
  * Note that you need at least 8Gb of RAM to run the PSD on the entire file. If you don't or if you get "Out of memory" errors, you can try running the PSD on a shorter time window.

 * Click on '''[Edit]''' and select option "'''Save individual PSD values''' (for each trial)".<<BR>><<BR>> {{attachment:psd1.gif||height="394",width="615"}}
 * Double-click on the new PSD files to display them.
 * Observations for '''Run''''''01''': <<BR>><<BR>> {{attachment:psd_eval01.gif||height="207",width="394"}}
  * Peaks related with the power lines: '''60Hz, 120Hz, 180Hz  '''(240Hz and 300Hz could be observed as well depending on the window length used for the PSD)

  * The drop after '''600Hz''' corresponds to the low-pass filter applied at the acquisition time.
  * One channel indicates a higher noise than the others in high frequencies: '''MLO52''' (in red).<<BR>>We will probably mark it as bad later, when reviewing the recordings.
 * Observations for '''Run02''': <<BR>><<BR>> {{attachment:psd_eval02.gif||height="208",width="394"}} <<BR>> {{attachment:psd_eval02_zoom.gif||height="208",width="595"}}
  * Same peaks related with the power lines:  '''60Hz, 120Hz, 180Hz'''
  * Same drop after '''600Hz'''.
  * Same noisy channel: '''MLO52'''.
  * Additionally, we observe higher level of noise in frequencies in the range of 30Hz to 100Hz on '''many occipital sensors'''. This is probably due to some tension in the neck due to an uncomfortable position. We will see later whether those channels need to be tagged as bad.

=== Power line contamination ===
 * Put '''ALL''' the "Link to raw file" into the Process1 box (or directly the Subject01 folder)
 * Run the process: '''Pre-process > Notch filter'''
  * Select the frequencies: '''60, 120, 180 Hz'''
  * Sensor types or names: '''MEG'''
  * The higher harmonics are too high to bother us in this analysis, plus they are not clearly visible in all the recordings.
  * In output, this process creates new .ds folders in the same folder as the original files, and links the new files to the database.<<BR>><<BR>> {{attachment:psd3.gif}}
 * Run again the PSD process "'''Frequency > Power spectrum density (Welch)'''" on those new files, with the same parameters, to evaluate the quality of the correction.
 * Double-click on the new PSD files to open them.<<BR>><<BR>> {{attachment:psd5.gif||height="177",width="416"}}
 * Zoom in with the mouse wheel to observe what is happening around 60Hz (before / after).<<BR>><<BR>> {{attachment:psd6.gif||height="143",width="453"}}
 * To avoid the confusion later, delete the links to the original files: Select the folders containing the original unfiltered files and press the Delete key (or right-click > File > Delete).<<BR>><<BR>> {{attachment:psd7.gif||height="184",width="356"}}

=== Heartbeats and eye blinks ===
 * Select the two AEF runs in the Process1 box.
 * Select successively the following processes, then click on [Run]:
  * '''Events > Detect  heartbeats:''' Select channel '''ECG''', check "All file", event name "cardiac".
  * '''Events > Detect eye blinks:''' Select channel '''VEOG''', check "All file", event name "blink".
  * '''Events > Remove simultaneous''': Remove "'''cardiac'''", too close to "'''blink'''", delay '''250ms'''.
  * '''Compute SSP: Heartbeats''': Event name "cardiac", sensors="MEG", '''do not use existing SSP'''.
  * '''Compute SSP: Eyeblinks''': Event name "blink", sensors="MEG", '''do not use existing SSP'''.<<BR>><<BR>> {{attachment:ssp_pipeline.gif||height="359",width="527"}}
 * Double-click on '''Run01 '''to open the MEG.<<BR>>You can change the color of events "standard" and "deviant" to make the figure more readable.
 * Review the '''EOG '''and '''ECG '''channels and make sure the events detected make sense.<<BR>><<BR>> {{attachment:events.gif||height="369",width="557"}}
 * In the Record tab, menu SSP > '''Select active projectors'''.
  * Blink: The first component is selected and looks good.
  * Cardiac: The category is disabled because no component has a value superior to 12%.
  * Select the first component of the cardiac category and display its topography.
  * It looks exactly like a cardiac topography, keep it selected and click on [Save].<<BR>><<BR>> {{attachment:ssp_result.gif||height="172",width="700"}}
 * Repeat the same operations for '''Run02''':
  * Review the events.
  * Select the first cardiac component.

=== Bad segments ===
 * At this point, you should review the entire files, by pages of a few seconds scrolling with the F3 key, to identify all the bad channels and the noisy segments of recordings. Do this with the the EOG channel open at the same time to identify saccades or blinks that were not completely corrected with the SSP projectors. As this is a complicated task that requires some expertise, we have prepared a list of bad segments for these datasets.
 * Open '''Run01'''. In the Record tab, select '''File > Add events from file''':
  * File name: sample_auditory/data/S01_AEF_20131218_01_notch/'''events_bad_01.mat '''
  * File type: Brainstorm (events*.mat)
  * It adds '''12 bad segments''' to the file.

 * Open '''Run02'''. In the Record tab, select '''File > Add events from file''':
  * File name: sample_auditory/data/S01_AEF_20131218_02_notch/'''events_bad_02.mat '''
  * File type: Brainstorm (events*.mat)
  * It adds '''9 bad segments''' and '''16 saccades''' to the file.

=== Saccades ===
 * Run02 contains a few saccades that generate a large amount of noise in the MEG recordings. They are not identified well by the automatic detection process based on the horizontal EOG. We have marked some of them, you have already loaded those events together with the bad segments. We are going to use again the SSP technique to remove the spatial components associated with those saccades.
 * Open the MEG recordings for '''Run02''' and select the right-frontal sensors (Record tab > CTF RF).
 * In the Record tab, menu SSP > Compute SSP: Generic<<BR>>Event name='''saccade''', Time='''[0,500]ms''', Frequency='''[1,15]Hz''', '''Use existing SSP'''<<BR>><<BR>> {{attachment:ssp_saccade_process.gif||height="442",width="329"}}
 * Example of saccade without correction:<<BR>><<BR>> {{attachment:ssp_saccade_before.gif||height="259",width="711"}}
 * With the first component of saccade SSP applied: <<BR>><<BR>> {{attachment:ssp_saccade_after.gif||height="283",width="507"}}
 * This first component removes really well the saccade, keep it selected and click on [Save].

=== Bad channels ===
 * During the visual exploration, some channels appeared generally noisier than the others. Example: <<BR>><<BR>> {{attachment:badchannel02.gif||height="200",width="456"}}
 * Right-click on '''Run01 '''> Good/bad channels > Mark some channels as bad<<BR>> > '''MRT51, MLO52'''
 * Right-click on '''Run02 '''> Good/bad channels > Mark some channels as bad<<BR>> > '''MRT51, ''''''MLO52, MLO42, MLO43'''

== Epoching and averaging ==
=== Import recordings ===
To import epochs from '''Run01''':

 * Right-click on the "Link to raw file" > '''Import in database'''

 * Use events: "'''standard'''" and "'''deviant'''"
 * Epoch time: '''[-100, +500] ms'''
 * Apply the existing SSP (make sure that you have 2 selected projectors)
 * '''Remove DC''' '''offset '''based on time window: '''[-100, 0] ms'''
 * '''UNCHECK''' the option "Create new conditions for epochs", this way all the epochs are going to be saved in the same Run01 folder, and we will able to separate the trials from Run01 and Run02.<<BR>><<BR>> {{attachment:import1.gif||height="344",width="521"}}
 * Note that the trials that are overlapping with a BAD segment are tagged as bad in the database explorer (marked with a red dot).<<BR>><<BR>> {{attachment:import2.gif}}

Repeat the same operation for '''Run02''':

 * Right-click on the "Link to raw file" > '''Import in database'''
 * Use the same options as for the previous run.<<BR>><<BR>> {{attachment:import3.gif||height="235",width="216"}}

=== Average responses ===
 * As said previously, it is usually not recommended to average recordings in sensor space across multiple acquisition runs because the subject might have moved between the sessions. Different head positions were recorded for each run, we will reconstruct the sources separately for each each run to take into account those movements.
 * However, in the case of event-related studies it makes sense to start our data exploration with an average across runs, just to evaluate the quality of the evoked responses. We have seen that the subject almost didn't move between the two runs, so the error would be minimal. We will compute now an approximate sensor average between runs, and we will run a more formal average in source space later.
 * We have 80 good "deviant" trials that we want to average together.
 * Select the trial groups "deviant" from both runs in Process1, run process "'''Average > Average files'''"<<BR>>Select the option "'''By trial group (subject average)'''"<<BR>><<BR>> {{attachment:process_average_data1.gif||height="456",width="470"}}
 * To compare properly this "deviant" average with the other condition, we need to use the same number of trials in the "standard" condition. We are going to pick 40 "standard" trials from Run01 and 40 from Run02. To make it easy, let's take the 40 first good trials.
 * Select the '''41 '''first "standard" trials of Run01 + the '''41 '''first "standard" trials of Run02 in Process1.<<BR>>This will sum to '''80 '''selected files, because the Process1 tab ignores the bad trials (trial #37 is bad in Run01, trial #36 is bad in Run02)
 * Run again process  "'''Average > Average files'''" > "'''By trial group (subject average)'''"<<BR>><<BR>> {{attachment:process_average_data2.gif||height="444",width="471"}}
 * The average for the two conditions "standard" and "deviant" are saved in the folder ''(intra-subject)''. The channel file added to this folder is an average of the channel files from Run01 and Run02.<<BR>><<BR>> {{attachment:average_sensor_files.gif||height="205",width="220"}}

=== Visual exploration ===
 * Display the two averages, "standard" and "deviant":
  * Right-click on average > MEG > Display time series
  * Right-click on average > MISC > Display time series (EEG electrodes Cz and Pz)
  * Right-click on average > MEG > 2D Sensor cap
  * In the Filter tab, add a '''low-pass filter''' at '''100Hz'''.
  * Right-click on the 2D topography figures > Snapshot > Time contact sheet.
 * Here are results for the standard (top) and deviant (bottom) beeps:<<BR>><<BR>> {{attachment:average_sensor.gif||height="399",width="703"}}
  * '''P50''': 50ms, bilateral auditory response in both conditions.
  * '''N100''': 95ms, bilateral auditory response in both conditions.
  * '''MMN''': 100-200ms, mismatch negativity in the deviant condition only (detection of deviant).
  * '''P200''': 170ms, in both conditions but much stronger in the standard condition.
  * '''P300''': 300-400ms, deviant condition only (decision making in preparation of the button press).
 * '''Standard '''(right-click on the topography figure > Snapshot > Time contact sheet) : <<BR>><<BR>>  {{attachment:average_sensor_standard.gif||height="286",width="370"}}
 * '''Deviant''': <<BR>><<BR>>  {{attachment:average_sensor_deviant.gif||height="300",width="371"}}

== Source estimation ==
=== Head model ===
 * Select the two imported folders at once, right-click > Compute head model<<BR>><<BR>> {{attachment:headmodel1.gif||height="221",width="413"}}
 * Use the '''overlapping spheres''' model and keep all of the options at their default values.<<BR>><<BR>> {{attachment:headmodel2.gif||height="234",width="209"}} {{attachment:headmodel3.gif||height="208",width="244"}}
 * For more information: [[Tutorials/TutHeadModel|Head model tutorial]].

=== Noise covariance matrix ===
 * We want to calculate the noise covariance from the empty room measurements and use it for the other runs.
 * In the '''Noise''' folder, right-click on the Link to raw file > Noise covariance > Compute from recordings.<<BR>><<BR>> {{attachment:noisecov1.gif||height="253",width="392"}}
 * Keep all the default options and click [OK]. <<BR>><<BR>> {{attachment:noisecov2.gif||height="285",width="291"}}
 * Right-click on the noise covariance file > Copy to other conditions.<<BR>><<BR>> {{attachment:noisecov3.gif||height="181",width="232"}}
 * You can double-click on one the copied noise covariance files to check what it looks like:<<BR>><<BR>> {{attachment:noisecov4.gif||height="232",width="201"}}
 * For more information: [[Tutorials/TutNoiseCov|Noise covariance tutorial]].

=== Inverse model ===
 * Select the two imported folders at once, right-click > Compute sources<<BR>><<BR>> {{attachment:inverse1.gif||height="192",width="282"}}
 * Select '''dSPM''' and keep all the default options.<<BR>><<BR>> {{attachment:inverse2.gif||height="205",width="199"}}
 * Then you are asked to confirm the list of bad channels to use in the source estimation for each run. Just leave the defaults, which are the channels that we set as bad earlier.<<BR>><<BR>> {{attachment:inverse_bad1.gif||height="202",width="326"}}
 * One inverse operator is created in each condition, with one link per data file.<<BR>><<BR>> {{attachment:inverse3.gif||height="240",width="202"}}
 * For more information: [[Tutorials/TutSourceEstimation|Source estimation tutorial]].

=== Average in source space ===
 * Now we have the source maps available for all the trials, we average them in source space.
 * Select the folders for '''Run01 '''and '''Run02 '''and the  ['''Process sources'''] button on the left.
 * Run process "'''Average > Average files'''":<<BR>>Select "'''By trial group (subject average)'''"<<BR>><<BR>> {{attachment:process_average_results.gif||height="376",width="425"}}
 * Double-click on the source averages to display them (standard=top, deviant=bottom).<<BR>><<BR>> {{attachment:average_source.gif||height="321",width="780"}}
 * Note that opening the source maps can be very long because of the online filters. Check in the Filter tab, you probably still have a '''100Hz low-pass filter''' applied for the visualization. In the case of averaged source maps, the 15000 source signals are filtered on the fly when you load a source file. This can take a significant amount of time. You may consider unchecking this option if the display is too slow on your computer.

 * Right-click on the 3D figures > Snapshot > Time contact sheet.<<BR>><<BR>>ADD 4 CONTACT SHEETS (2 conditions x 2 orientations)

 * Right-click on any figure > Snapshot > Movie (time): All figures<<BR>><<BR>>{attachment:average_source_video.gif}

<<HTML(<video width="320" height="240" controls><source src="http://neuroimage.usc.edu/brainstorm/wikidocs/average_sources.avi" type="video/mp4"> Your browser does not support the video tag.</video>)>>

== Regions of interest [TODO] ==
Scouts over primary auditory cortex (A1)

Standard:

{{attachment:beth_standard_sources.png||height="357",width="530"}}

Deviant:

{{attachment:beth_deviant_sources.png||height="356",width="530"}}

Deviant-standard:

=> Averaged the deviant runs from Run01 and Run02 (76 trials), then averaged 38 standard trials from Run01 with 38 standard trials from Run02 (76 files), subtracted the standard from the deviant and computed sources and z-score.

=> ACC activity around 300ms - this indicates error detection.

{{attachment:beth_deviant-standard_acc.png||height="185",width="454"}}

== Time-frequency [TODO] ==
TF over scout 1, z-scored [-100,0] ms

Interesting gamma in the deviant:

{{attachment:beth_tf.png||height="328",width="224"}}

== Coherence [TODO] ==
 * Run01, sensor magnitude-square coherence 1xN
  * UADC001 vs. other sensors
  * Time window: [0,150]ms
  * Sensor types
  * Remove evoked response: NOT SELECTED
  * Maximum frequency resolution: 1Hz
  * Highest frequency of interest: 80Hz
  * Metric significativity: 0.05
  * Concatenate input files: SELECTED
  * => Coh(0.6Hz,32win)<<BR>><<BR>> {{attachment:beth_coherence_sensor_process.png||height="369",width="292"}}
  * At 13Hz<<BR>><<BR>> {{attachment:beth_coherence_sensor_200trials_13Hz.png||height="191",width="341"}}
 * Run01, source magnitude-square coherence 1xN
  * 50 standard trials
  * Highest frequency of interest: 40Hz
  * Same other inputs<<BR>><<BR>> {{attachment:beth_coherence_source_settings_run2_0-150ms.png||height="408",width="262"}}
  * At 13Hz:<<BR>><<BR>> {{attachment:beth_coherence_source_50trials_[0,150]ms_13Hz.png|beth_coherence_source_50trials_[0,150]ms_13Hz.png|height="304",width="388"}}

== Discussion ==
Discussion about the choice of the dataset on the !FieldTrip bug tracker:<<BR>>http://bugzilla.fcdonders.nl/show_bug.cgi?id=2300

== Scripting [TODO] ==
==== Process selection ====
==== Graphic edition ====
==== Generate Matlab script ====
The operations described in this tutorial can be reproduced from a Matlab script, available in the Brainstorm distribution: '''brainstorm3/toolbox/script/tutorial_auditory.m '''

== Feedback ==
<<EmbedContent(http://neuroimage.usc.edu/bst/get_feedback.php?Tutorials/Auditory)>>