Yokogawa/KIT tutorial
[TUTORIAL UNDER DEVELOPMENT: NOT READY FOR PUBLIC USE]
Authors: Francois Tadel, Yasuhiro Haruta, Ei-ichi Okumura, Takashi Asakawa.
This tutorial introduces some concepts that are specific to the management of MEG/EEG files recorded with Yokogawa/KIT systems in the Brainstorm environment.
Contents
License
This tutorial dataset (MEG/EEG and MRI data) remains proprietary of Yokogawa Electric Corporation, Japan. Its use and transfer outside the Brainstorm tutorial, e.g. for research purposes, is prohibited without written consent from Yokogawa Electric Corporation.
Description of the experiment
This tutorial is based on a simple median nerve stimulation experiment:
- Unilateral median nerves were percutaneously stimulated using monophasic square-wave impulses with a duration of 0.3 ms at 2.8 Hz.
- The stimulus intensity was set at the motor threshold to evoke mild twitches of the thumb.
- The stimulus onsets were recorded as low-to-high TTL with a trigger channel labeled as "Trigger01".
- The total number of stimuli in the dataset was 336.
- The data was recorded with a Yokogawa 160 axial gradiometer system at Yokogawa Electric Corporation, Kanazawa, Japan.
Export recordings from Meg160
To import Yokogawa/KIT data files (.con, .raw, .ave) into Brainstorm, a data export process is required beforehand. The data export function is available in Meg160, which is data analysis software equipped in most of Yokogawa/KIT systems.
The dataset used in this tutorial has already been exported using this procedure. It is described here so that later you can export your own recordings to Brainstorm.
If your software does not support the functions used below, please contact Yokogawa via
http://www.yokogawa.com/me/index.htm
Export the digitizer file
- If a wave data file and the corresponding digitizer file are ready for use, there is no additional operation required.
- If no digitizer file is available, you need first to extract the head surface points:
In Meg160, select the menu: File > Import and Export > BESA Text Export > Surface Point File
- Check that the fiducial points are properly pointed, and then click the [OK] button
- A surface point file (.sfp) is automatically created. It includes the position data of: the fiducial points, the marker points and other points describing the head shape
Export the recordings
In Meg160, select the menu: File > Import and Export > Third-Party Export
- On some systems, this menu is name [BESA Binary Export]
- An operation panel for data export shows up
- When using digitizer data:
- Select [Digitizer]
Enter the digitizer file in the [Point Filename] box
(.txt file generally available under the corresponding "Scan" folder)Enter the label file in the [Label Filename] box
(DigitizeLabel.txt generally located in the "C:\Meg160\AppInfo" folder)
- When using surface point data instead:
- Select [Surface Point File]
- Enter the surface point file (.sfp) created previously.
- Enter an output file name in [Third-party Export Dataset]
- Click on [Create Export File]
- Use this exported file in Brainstorm
Download and installation
- Requirements: You have already followed all the introduction tutorials and you have a working copy of Brainstorm installed on your computer.
Go to the Download page of this website, and download the file: sample_yokogawa.zip
Unzip it in a folder that is not in any of the Brainstorm folders (program or database folder)
- Start Brainstorm (Matlab scripts or stand-alone version)
Select the menu File > Create new protocol. Name it "TutorialYokogawa" and select the options:
"No, use individual anatomy",
"No, use one channel file per condition".
Import the anatomy
Right-click on the TutorialYokogawa folder > New subject > Subject01
- Leave the default options you set for the protocol
Right-click on the subject node > Import anatomy folder:
Set the file format: "FreeSurfer folder"
Select the folder: sample_yokogawa/anatomy
- Number of vertices of the cortex surface: 15000 (default value)
- Set the 6 required fiducial points (indicated in MRI coordinates):
- NAS: x=128, y=227, z=93
- LPA: x=48, y=130, z=69
- RPA: x=214, y=130, z=76
- AC: x=128, y=148, z=116
- PC: x=128, y=123, z=117
- IH: x=128, y=131, z=176 (anywhere on the midsagittal plane)
At the end of the process, make sure that the file "cortex_15000V" is selected (downsampled pial surface, which will be used for the source estimation). If it is not, double-click on it to select it as the default cortex surface.
Without the individual MRI
If you do not have access to an individual MR scan of the subject (or if its quality is too low to be processed with FreeSurfer), but if you have digitized the head shape of the subject using a tracking system, you have an alternative: deform one of the Brainstorm templates (Colin27 or ICBM152) to match the shape of the subject's head.
For more information, read the following tutorial: Warping default anatomy
Access the recordings
Link the recordings
- Switch to the "functional data" view.
Right-click on the subject folder > Review raw file:
- Select the file format: "MEG/EEG : Yokogawa/KIT"
Select the file: sample_yokogawa/data/SEF_000-export.con
Answer NO when asked to refine the registration using head points. In this dataset, we only have access to the positions of the electrodes and three additional markers on the forehead. The automatic registration doesn't work well in this case, we are going to fix this registration manually.
- A figure is opened to show the current registration MRI/MEG. It is already quite good, but can be improved a bit manually. Close this figure.
- The new file "Link to raw file" lets you access directly the contents of the MEG/EEG recordings
- The channel file "KIT channels" contains the name of the channels and the position of the corresponding sensors.
Prepare the channel file
- The recordings contain signals coming from different types of electrodes:
- 160 MEG channels
- 12 MEG references
- 14 Trigger channels
- 41 EEG channels
- 2 EOG channels: EO1 and EO2
- 1 ECG channel: EKG+
- 1 ground of the EEG amplifier: E
- Not all the types of channels are properly identified in Brainstorm. We need to redefine this manually to get correct groups of sensors.
Right-click on the channel file > Edit channel file:
Channel EO1 (208) and EO2 (209): Change the type to EOG
Channel EKG+ (214): Change the type to ECG
Channel E (231): Change the type to MISC
- Close the figure and accept to save the modifications
Refine the MRI registration
Right-click on the channel file > MRI registration > Edit... (EEG)
- The white points are the electrodes, the green points are the additional digitized head points. To display the label of the electrodes, click on the [LABEL] button in the toolbar. To see what the other buttons in the toolbar are doing and how to use them, leave your mouse over them for a few seconds and read the description.
Now try to manipulate the position of the EEG+MEG sensors using rotations and translations only (no "resize" or individual electrodes adjustments). The objective is to have all the points close to the surface and the three forehead points inside the little peaks on the surface (due to markers in the MRI).
The rotation+translation are going to be applied both to the EEG and the MEG sensors. After you are done with this solid registration part, you can click on the button "Project electrodes on scalp surface", it will help for the source modeling later. The green points (digitized) stay in place, the white points (electrodes) are now projected on the skin of the subject.
- If you feel like you made it wrong, close the figure and cancel the modifications, then try again. It takes a few trials to get used to this rotation/translation interface.
Click on [OK] when done.
Answer YES to save the modifications.
Answer YES again to apply the solid transformation (rotation+translation) to the MEG sensors.
Before manual registration:
After manual registration:
Read the stimulation information
Right-click on the "Link to raw file" > Trigger > Display time series
- In the Record tab, switch to a column view of the sensors (first button in the toolbar)
You can see that all the trigger lines are flat except for "Trigger01", which contains the information of the electric stimulation. We are going to read this trigger channel and convert it to a list of events. Close this figure.
- Drag and drop the "Link to raw file" in the Process1 box.
Run the process "Import recordings > Read from channel"
Event channel = Trigger01
Option selected "TTL": detect peaks of 5V/12V on an analog channel
Do not select the option Accept zeros as trigger values
Right-click on the "Link to raw file" > Trigger > Display time series. Check that the peaks of the triggers channel have correctly been identified, then close this figure.
Artifacts: Evaluate the power spectrum
Evaluation
Two of the typical pre-processing steps consist in getting rid of the contamination due to the power lines (50 Hz or 60Hz) and of the frequencies we are not interested in (a low-pass filter to remove the high-frequencies and a high-pass filter to remove the very slow components of the signals). Let's start with the spectral evaluation of this file.
Drag the "Link to raw file" to the Process1 box and run the process "Frequency > Power spectrum density (Welch)". Configure it as illustrated in the following figure (window length=10s, overlap=50%).
Double-click on the new PSD file to display it.
- The lines on the top represent the EEG electrodes, the lines at the bottom the MEG sensors. If you want to get clearer plots, you can calculate separately the spectrum for the two types of sensors separately, by running twice the process "Power spectrum density" , once with sensor types = "MEG" and once with "EEG", instead of running in on both at the same time like we did.
- Observations (below 250Hz):
- Peak around 11Hz: Alpha waves from the subject's brain
Peaks at 60Hz, 120Hz, 180Hz, 240Hz on EEG + MEG:
Electric contamination due to the power lines (60Hz+harmonics)Peaks at 35Hz, 65Hz, 70Hz, 183Hz, 197Hz on MEG only:
Electric noise coming from an unknown sourceMEG sensor LC11 appears to have a higher level of noise than all the other MEG sensors, we will check this when review the MEG recordings and probably tag it as a bad channel.
Correction
- From the Yokogawa software:
- Users can apply digital filtering to the data before exporting them to Brainstorm. Depending on the data size and degrees of filter parameters, it sometime takes a very long time.
- From Brainstorm:
There is no easy way to process the Yokogawa files at this time using the Brainstorm software. This capability might be added in the future, in which case the procedure would be very similar to the one presented in the basic tutorial ?Detect and remove artifacts.
For short recordings, if the contamination is really bad, there is an alternative option: importing the entire file in Brainstorm and then process it. This approach is illustrated in the EEG/Epilepsy tutorial. This approach requires a very large amount of memory, it is not recommended on MEG recordings as the files tend to be much bigger than EEG-only recordings.
- For now we are going to keep the file as it is. There is no important contamination below 60Hz and we are going to study processes that occur at lower frequencies. When studying evoked responses, the electric noise at 60Hz tends to cancel out and is usually not a problem in the analysis. Note for later that it is however important to consider this electric noise when working on resting state recordings or time-frequency decompositions at higher frequencies.
Artifacts: Heartbeats and eye blinks [TO DO]
Run process "Artifacts > Detect eye blinks" on channel EOG1 and/or EOG2
Run process "Artifacts > Detect heartbeat" on channel EKG+
Run process "Artifacts > SSP: Eye blinks" for MEG and/or EEG
Run process "Artifacts > SSP: Heartbeat" for MEG and/or EEG
Review the recordings
- MEG:
Right-click on the "Link to raw file" > MEG > Display time series.
- Display the channels in columns (first button in the toolbar of the Record tab)
Click on the noisy LC11 sensor to select it (displayed in red)
Right-click in the figure > Channels > Mark selected as bad
- Close this figure
Right-click on the "Link to raw file" > EEG > Display time series.
- We are not going to detail more in details the interface to review MEG or EEG recordings as it is already done in other specialized tutorials:
For continuous MEG: ?Review continuous recordings and edit markers
For continuous EEG: EEG and epilepsy
For imported files: ?Exploring the recordings
Epoching and averaging
Import recordings
In this experiment, the electric stimulation is sent with a frequency of 2.8Hz, meaning that the inter-stimulus interval is 357ms. We are going to import epochs of 300ms around the stimulation events.
Right-click on the Link to raw file > Import in database:
- Select the entire time definition (0s to 120s, default)
Check "Use events" and select "Trigger01"
Epoch time: [-50, 250] ms
Check "Remove DC offset" > Time range > [-50, -10] ms
SCREEN CAPTURE
At the end, you are asked whether you want to ignore one epoch that is shorter than the others. This happens because the acquisition of the MEG signals was stopped less than 250ms after the last stimulus trigger was sent. Therefore, the last epoch cannot have the full [-50,250]ms time definition. This shorter epoch would prevent us from averaging all the trials easily. As we already have enough repetitions in this experiment, we can just ignore it.
Answer Yes to this question to discard the last epoch.
Average epochs
Source analysis
Head model
Noise covariance matrix
Inverse model
Z-score normalization
Regions of interest
Scripting
Graphic edition
Generate Matlab script
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