= MEG visual tutorial: Single subject (original) =
''Authors: Francois Tadel, Elizabeth Bock. ''

The aim of this tutorial is to reproduce in the Brainstorm environment the analysis described in the SPM tutorial "[[ftp://ftp.mrc-cbu.cam.ac.uk/personal/rik.henson/wakemandg_hensonrn/Publications/SPM12_manual_chapter.pdf|Multimodal, Multisubject data fusion]]". The data processed here consists of simultaneous MEG/EEG recordings from 19 participants performing a simple visual recognition task from presentations of famous, unfamiliar and scrambled faces.

The analysis is split in two tutorial pages: the present tutorial describes the detailed analysis of one single subject; the second tutorial describes batch processing and [[Tutorials/VisualGroup|group analysis of all 19 participants]].

Note that the operations used here are not detailed, the goal of this tutorial is not to introduce Brainstorm to new users. For in-depth explanations of the interface and theoretical foundations, please refer to the [[http://neuroimage.usc.edu/brainstorm/Tutorials#Get_started|introduction tutorials]].

'''WARNING''': This tutorial corresponds to the first version of the openfmri dataset described below. A new tutorial is [[Tutorials/VisualSingle|available here]] to handle the BIDS version of the dataset.

<<TableOfContents(3,2)>>

== License ==
This dataset was obtained from the OpenfMRI project (http://www.openfmri.org), accession #ds117. It is made available under the Creative Commons Attribution 4.0 International Public License. Please cite the following reference if you use these data: <<BR>>Wakeman DG, Henson RN, [[http://www.nature.com/articles/sdata20151|A  multi-subject, multi-modal human neuroimaging dataset]], Scientific Data (2015)

Any questions regarding the data, please contact: rik.henson@mrc-cbu.cam.ac.uk

== Presentation of the experiment ==
==== Experiment ====
 * 19 subjects (three were excluded from the group analysis for [[http://neuroimage.usc.edu/brainstorm/Tutorials/VisualSingle#Bad_subjects|various reasons]])
 * 6 acquisition runs (aka sessions) of approximately 10mins for each subject
 * Presentation of series of images: familiar faces, unfamiliar faces, phase-scrambled faces
 * Participants had to judge the left-right symmetry of each stimulus
 * Total of nearly 300 trials for each of the 3 conditions

==== MEG acquisition ====
 * Acquisition at 1100Hz with an Elekta-Neuromag VectorView system (simultaneous MEG+EEG).
 * Recorded channels (404):
  * 102 magnetometers
  * 204 planar gradiometers
  * 70 EEG electrodes recorded with a nose reference.
 * MEG data have been "cleaned" using Signal-Space Separation as implemented in MaxFilter 2.2.
 * A  Polhemus device was used to digitize three fiducial points and a  large number of other points across the scalp, which can be used to  coregister the M/EEG data with the structural MRI image.
 * Stimulation triggers: The triggers related with the visual presentation are saved in the STI101 channel, with the following event codes (bit 3 = face, bit 4 = unfamiliar, bit 5 = scrambled):
  * Famous faces: 5 (00101), 6 (00110), 7 (00111)
  * Unfamiliar faces: 13 (01101), 14 (01110), 15 (01111)
  * Scrambled images: 17 (10001), 18 (10010), 19 (10011)
  * Delays between the trigger in STI101 and the actual presentation of stimulus: '''34.5ms'''
 * The data distribution includes MEG noise recordings acquired around the dates of the experiment, processed with MaxFilter 2.2 in the same way as the experimental data.

==== Subject anatomy ====
 * MRI data acquired on a 3T Siemens TIM Trio: 1x1x1mm T1-weighted structural MRI.
 * The face was removed from the strucural images for anonymization purposes.
 * Processed with FreeSurfer 5.3.

== Download and installation ==
 * First, make sure you have enough space on your hard drive, at least '''350Gb''':
  * Raw files: '''100Gb'''
  * Processed files: '''250Gb'''
 * The data is hosted on the OpenfMRI website: https://openfmri.org/dataset/ds000117/
 * Download all the files available from this website (approximately 160Gb):<<BR>>'''ds117_metadata.tgz''', '''ds117_sub001_raw.tgz''', ..., '''ds117_sub019_raw.tgz'''

 * Unzip all the .tgz files in the same folder.
 * The FreeSurfer segmentations of the T1 images are not part of the OpenfMRI distribution. You can either process them by yourself, or download the result  of the segmentation from the Brainstorm website. <<BR>>Go to the [[http://neuroimage.usc.edu/bst/download.php|Download]] page, and download the file: '''sample_group_freesurfer.zip'''<<BR>>Unzip this file in the same folder as the other files.
 * We will use the following files from this distribution:
  * '''/anatomy/subXXX/''': Segmentation folders generated with FreeSurfer.
  * '''/emptyroom/090707_raw_st.fif''': MEG empty room measurements, processed with MaxFilter.
  * '''/ds117/subXXX/MEG/*_sss.fif''': MEG and EEG recordings, processed with MaxFilter.
  * '''/README''': License and dataset description.
 * Reminder: Do not save the downloaded files in the Brainstorm  folders (program or database folders).
 * Start Brainstorm (Matlab scripts or stand-alone version). For help, see the [[Installation]] page.

 * Select the menu File > Create new protocol. Name it "'''TutorialVisual'''" and select the options:
  * "'''No, use individual anatomy'''",
  * "'''No, use one channel file per condition'''".

== Import the anatomy ==
This page explains how to import and process subject '''#002''' only. Subject #001 was not the best example because it will be later excluded from the EEG group analysis (incorrect electrodes positions).

 * Switch to the "anatomy" view.
 * Right-click on the TutorialVisual folder > New subject > '''sub002'''
  * Leave the default options you defined for the protocol.
 * Right-click on the subject node > Import anatomy folder:
  * Set the file format: "FreeSurfer folder"
  * Select the folder: '''anatomy/freesurfer/sub002'''  (from sample_group_freesurfer.zip)
  * Number of vertices of the cortex surface: 15000 (default value)
 * The two sets of fiducials we usually have to define interactively are here automatically set.
  * '''NAS/LPA/RPA''': The file Anatomy/Sub01/fiducials.m contains the definition of the nasion, left and right ears. The anatomical points used by the authors are the same as the ones we recommend in the Brainstorm [[CoordinateSystems|coordinates systems page]].
  * '''AC/PC/IH''': Automatically identified using the SPM affine registration with an MNI template.
  * If you want to double-check that all these points were correctly marked after importing the anatomy, right-click on the MRI > Edit MRI.

 * At   the end of the process, make sure that the file "cortex_15000V" is   selected (downsampled pial surface, that will be used for the source   estimation). If it is not, double-click on it to select it as the   default cortex surface. Do not worry about the big holes in the head surface, parts of MRI have been remove voluntarily for anonymization purposes.<<BR>><<BR>> {{attachment:anatomy_import.gif||height="384",width="613"}}

 * All the anatomical atlases [[Tutorials/LabelFreeSurfer|generated by FreeSurfer]] were automatically imported: the cortical atlases (Desikan-Killiany, Mindboggle, Destrieux, Brodmann) and the sub-cortical regions (ASEG atlas). <<BR>><<BR>> {{attachment:anatomy_atlas.gif||height="211",width="550"}}

== Access the recordings ==
=== Link the recordings ===
We need to attach the continuous .fif files containing the recordings to the database.

 * Switch to the "functional data" view.
 * Right-click on the subject folder > '''Review raw file'''.
  * Select the file format: "'''MEG/EEG: Neuromag FIFF (*.fif)'''"
  * Select the first file in the MEG folder for subject 002: '''sub002/MEEG/run_01_sss.fif''' <<BR>><<BR>> {{attachment:review_raw.gif||height="202",width="542"}}
 * Events:''' Ignore'''. We will read the stimulus triggers later.<<BR>><<BR>> {{attachment:review_ignore.gif||height="186",width="330"}}
 * Refine registration now? '''NO''' <<BR>>The head points that are available in the FIF files contain all the points that were digitized during the MEG acquisition, including the ones corresponding to the parts of the face that have been removed from the MRI. If we run the fitting algorithm, all the points around the nose will not match any close points on the head surface, leading to a wrong result. We will first remove the face points and then run the registration manually.

=== Channel classification ===
A few non-EEG channels are mixed in with the EEG channels, we need to change this before applying any operation on the EEG channels.

 * Right-click on the channel file > '''Edit channel file'''. Double-click on a cell to edit it.
 * Change the type of '''EEG062''' to '''EOG '''(electrooculogram).
 * Change the type of '''EEG063 '''to '''ECG '''(electrocardiogram).
 * Change the type of '''EEG061''' and '''EEG064''' to '''NOSIG'''. Close the window and save the modifications. <<BR>><<BR>> {{attachment:channel_edit.gif||height="252",width="561"}}

=== MRI registration ===
At this point, the registration MEG/MRI is based only on the three anatomical landmarks NAS/LPA/RPA. All the MRI scans were anonymized (defaced) and for some subjects the nasion could not be defined properly. We will try to refine this registration using the additional head points that were digitized (only the points above the nasion).

 * Right-click on the channel file > '''Digitized head points > Remove points below nasion'''.<<BR>><<BR>> {{attachment:channel_remove.gif||height="194",width="329"}}
 * Right-click on the channel file > '''MRI registration > Refine using head points'''.<<BR>><<BR>> {{attachment:channel_refine.gif||height="173",width="294"}}
 * MEG/MRI registration, before (left) and after (right) this automatic registration procedure:  <<BR>><<BR>> {{attachment:registration.gif||height="209",width="236"}} {{attachment:registration_final.gif||height="208",width="237"}}
 * Right-click on the channel file > MRI registration > EEG: Edit...<<BR>>Click on ['''Project electrodes on surface'''], then close the figure to save the modifications.<<BR>><<BR>> {{attachment:channel_project.gif||height="207",width="477"}}

=== Read stimulus triggers ===
We need to read the stimulus markers from the STI channels. The following tasks can be done in an interactive way with menus in the Record tab, as in the introduction tutorials. We will illustrate here how to do this with the pipeline editor, it will be easier to batch it for all the runs and all the subjects.

 * In Process1, select the "Link to raw file", click on [Run].
 * Select process '''Events > Read from channel''', Channel: '''STI101''', Detection mode: '''Bit'''.<<BR>>Do not execute the process, we will add other processes to classify the markers.<<BR>><<BR>> {{attachment:raw_read_events.gif||height="290",width="499"}}

 * We want to create three categories of events, based on their numerical codes:
  * '''Famous faces''': 5 (00101), 6 (00110), 7 (00111) => Bit 3 only
  * '''Unfamiliar faces''': 13 (01101), 14 (01110), 15 (01111) => Bit 3 and 4
  * '''Scrambled images''': 17 (10001), 18 (10010), 19 (10011) => Bit 5 only
  * We will start by creating the category "Unfamiliar" (combination of events "3" and "4") and discard the original event categories. Then we need to rename the remaining "3" events into "Famous", and all the "5" events into "Scrambled".
 * Add process '''Events > Group by name''': "'''Unfamiliar=3,4'''", Delay=0, '''Delete original events'''
 * Add process '''Events > Rename event''': 3 => Famous
 * Add process '''Events > Rename event''': 5 => Scrambled <<BR>><<BR>> {{attachment:events_merge.gif||height="402",width="532"}}
 * Add process '''Events > Add time offset''' to compensate for the presentation delays:<<BR>>Event names: "'''Famous, Unfamiliar, Scrambled'''", Time offset = '''34.5ms'''<<BR>><<BR>> {{attachment:events_offset.gif||height="355",width="348"}}

 * Finally run the script. Double-click on the recordings to make sure the labels were detected correctly. You can delete the unwanted, original event categories that remain in the data (categories: 1,2,9,13): <<BR>><<BR>> {{attachment:events_display.gif||height="194",width="595"}}

== Pre-processing ==
=== Spectral evaluation ===
 * Keep the "Link to raw file" in Process1.
 * Run process '''Frequency > Power spectrum density (Welch)''' with the options illustrated below. <<BR>><<BR>> {{attachment:psd_process.gif||height="314",width="669"}}
 * Right-click on the PSD file > Power spectrum.  <<BR>><<BR>> {{attachment:psd_plot.gif||height="235",width="408"}}
 * The MEG sensors look awful, because of one small segment of data located around 248s. Open the MEG recordings and scroll to 248s (just before the first Unfamiliar event). <<BR>><<BR>> {{attachment:psd_error.gif||height="183",width="527"}}
 * In these recordings, the continuous head tracking was activated, but it starts only at the time of the stimulation (248s) while the acquisition of the data starts 20s before (226s). The first 20s do not have head localization coils (HPI) coils activity and are not corrected by MaxFilter. After 248s, the HPI coils are on, and MaxFilter filters them out. The transition between the two states is not smooth and creates important distortions in the spectral domain. For a proper evaluation of the recordings, we should '''compute the PSD only after the HPI coils are turned on'''.
 * Run process '''Events > Detect cHPI activity (Elekta)'''. This detects the changes in the cHPI activity from channel STI201 and marks all the data without head localization as bad. <<BR>><<BR>> {{attachment:psd_badsegment.gif||height="234",width="503"}}
 * Re-run the process  '''Frequency > Power spectrum density (Welch)'''. All the bad segments are excluded from the computation, therefore the PSD is now estimated only with the data '''after 248s'''.  <<BR>><<BR>> {{attachment:psd_fix.gif||height="283",width="668"}}
 * Observations:
  * Three groups of sensors, from top to bottom: EEG, MEG gradiometers, MEG magnetometers.
  * Power lines: '''50 '''Hz and harmonics
  * Alpha peak around 10 Hz
  * Artifacts due to Elekta electronics (HPI coils): '''293'''Hz, '''307'''Hz, '''314'''Hz, '''321'''Hz, '''328'''Hz.
  * Peak from unknown source at '''103.4Hz''' in the MEG only.
  * Suspected bad EEG channels: '''EEG016'''
 * Close all the windows.

=== Remove line noise ===
 * Keep the "Link to raw file" in Process1.
 * Select process '''Pre-process > Notch filter''' to remove the line noise (50-200Hz).<<BR>>Add the process '''Frequency > Power spectrum density (Welch)'''. <<BR>><<BR>> {{attachment:notch_process.gif||height="262",width="562"}}
 * Double-click on the PSD for the new continuous file to evaluate the quality of the correction. <<BR>><<BR>> {{attachment:notch_result.gif||height="215",width="566"}}
 * Close all the windows (use the [X] button at the top-right corner of the Brainstorm window).

=== EEG reference and bad channels ===
 * Right-click on link to the processed file ("Raw | notch(50Hz ...") > '''EEG > Display time series'''.
 * Select channel '''EEG016''' and mark it as '''bad''' (using the popup menu or pressing the Delete key). <<BR>><<BR>> {{attachment:channel_bad.gif||height="214",width="587"}}
 * In the Record tab, menu '''Artifacts > Re-reference EEG''' > "AVERAGE". <<BR>><<BR>> {{attachment:channel_ref.gif||height="270",width="535"}}
 * At the end, the window "select active projectors" is open to show the new re-referencing projector. Just close this window. To get it back, use the menu Artifacts > Select active projectors.

== Artifact detection ==
=== Heartbeats: Detection ===
 * Empty the Process1 list (right-click > Clear list).
 * Drag and drop the continuous processed file ("Raw | notch(50Hz...)") to the Process1 list.
 * Run process''' Events > Detect heartbeats''': Channel name='''EEG063''', All file, Event name=cardiac <<BR>><<BR>> {{attachment:detect_cardiac.gif||height="230",width="310"}}

=== Eye blinks: Detection ===
 * In many of the other tutorials, we detect the blinks and remove them with SSP. In this experiment, we are particularly interested in the subject's response to seeing the stimulus. Therefore we will exclude from the analysis all the recordings contaminated with blinks or other eye movements.

 * Run process '''Artifacts > Detect events above threshold''': <<BR>>Event name='''blink_BAD''', Channel='''EEG062''', All file,  Maximum threshold='''100''', Threshold units='''uV''', Filter='''[0.30,20.00]Hz''',  '''Use absolute value of signal'''.<<BR>><<BR>> {{attachment:detect_blinks.gif||height="455",width="285"}}

 * Inspect visually the two new cateogries of events: cardiac and blink_BAD.<<BR>><<BR>> {{attachment:detect_display.gif||height="237",width="655"}}

 * Close all the windows (using the [X] button).

=== Heartbeats: Correction with SSP ===
 * Keep the "Raw | notch" file selected in Process1.
 * Select process '''Artifacts > SSP: Heartbeats''' >  Sensor type: '''MEG MAG'''
 * Add process '''Artifacts > SSP: Heartbeats''' >  Sensor type: '''MEG GRAD''' - Run the execution <<BR>><<BR>> {{attachment:ssp_ecg_process.gif||height="225",width="293"}}
 * Double-click on the continuous file to show all the MEG sensors. <<BR>>In the Record tab, select sensors "Left-temporal".
 * Menu Artifacts > Select active projectors.
  * In category '''cardiac/MEG MAG''': Select '''component #1''' and view topography.
  * In category '''cardiac/MEG GRAD''': Select '''component #1 '''and view topography. <<BR>><<BR>> {{attachment:ssp_ecg_topo.gif||height="175",width="604"}}

 * Make sure that selecting the two components removes the cardiac artifact. Then click '''[Save]'''.

=== Additional bad segments ===
 * Process1>Run: Select the process "'''Events > Detect other artifacts'''". This should be done separately for MEG and EEG to avoid confusion about which sensors are involved in the artifact. <<BR>><<BR>> {{attachment:detect_other.gif||height="253",width="486"}}
 * Display the MEG sensors. Review the segments that were tagged as artifact, determine if each event represents an artifact and then mark the time of the artifact as BAD. This can be done by selecting the time window around the artifact, then right-click > '''Reject time segment'''. Note that this detection process marks 1-second segments but the artifact can be shorter.<<BR>><<BR>> {{attachment:artifacts_mark_bad.png||width="640"}}

 * Once all the events in the two categories are reviewed and bad segments are marked, the two categories (1-7Hz and 40-240Hz) can be deleted.
 * Do this detection and review again for the EEG.

=== SQUID jumps ===
MEG signals recorded with Elekta-Neuromag systems frequently contain SQUID jumps ([[http://neuroimage.usc.edu/brainstorm/Tutorials/BadSegments?highlight=(squid)#Elekta-Neuromag_SQUID_jumps|more information]]). These sharp steps followed by a change of baseline value are easy to identify visually but more complicated to detect automatically.

The process "Detect other artifacts" usually detects most of them in the category "1-7Hz". If you observe that some are skipped, you can try re-running it with a higher sensitivity. It is important to review all the sensors and all the time in each run to be sure these events are marked as bad segments.

 . {{attachment:detect_jumps.gif||height="214",width="547"}}

== Epoching and averaging ==
=== Import epochs ===
 * Keep the "Raw | notch" file selected in Process1.

 * Select process: '''Import > Import recordings > Import MEG/EEG: Events''' (do not run immediately)<<BR>>Event names "Famous, Unfamiliar, Scrambled", All file, Epoch time=[-500,1200]ms
 * Add process: '''Pre-process > Remove DC offset''': Baseline=[-500,-0.9]ms - Run execution.<<BR>><<BR>> {{attachment:import_epochs.gif||height="399",width="647"}}

=== Average by run ===
 * In Process1, select all the imported trials.
 * Run process: '''Average > Average files''': '''By trial groups (folder average)''' <<BR>><<BR>>  {{attachment:average_process.gif||height="504",width="478"}}

=== Review EEG ERP ===
 * EEG evoked response (famous, scrambled, unfamiliar): <<BR>><<BR>> {{attachment:average_topo.gif||height="392",width="684"}}
 * Open the [[Tutorials/ChannelClusters|Cluster tab]] and create a cluster with the channel '''EEG065''' (button [NEW IND]).<<BR>><<BR>> {{attachment:cluster_create.gif||height="166",width="569"}}
 * Select the cluster, select the three average files, right-click > '''Clusters time series''' (Overlay:Files). <<BR>><<BR>> {{attachment:cluster_display.gif||height="220",width="658"}}
 * Basic observations for EEG065 (right parieto-occipital electrode):
  * Around 170ms (N170): greater negative deflection for Famous than Scrambled faces.
  * After 250ms: difference between Famous and Unfamiliar faces.

== Source estimation ==
=== MEG noise covariance: Empty room recordings ===
The minimum norm model we will use next to estimate the source activity  can be improved by modeling the the noise contaminating the data. The  section shows how to [[Tutorials/NoiseCovariance|estimate the noise covariance]]  in different ways for EEG and MEG. For the MEG recordings we will use  the empty room measurements we have, and for the EEG we will compute it  from the pre-stimulus baselines we have in all the imported epochs.

 * Create a new subject: '''emptyroom'''
 * Right-click on the new subject > '''Review raw file'''.<<BR>>Select file: '''sample_group/emptyroom/090707_raw_st.fif '''<<BR>>Do not apply default transformation, Ignore event channel.<<BR>><<BR>> {{attachment:noise_review.gif||height="164",width="506"}}
 * Select this new file in Process1 and run process '''Pre-process > Notch filter''': '''50 100 150 200Hz'''. When using empty room measurements to compute the noise covariance, they must be processed exactly in the same way as the other recordings. <<BR>><<BR>> {{attachment:noise_notch.gif||height="225",width="436"}}
 * Right-click on the filtered noise recordings > '''Noise covariance > Compute from recordings''': <<BR>><<BR>> {{attachment:noise_compute.gif||height="240",width="649"}}
 * Right-click on the '''Noise covariance > Copy to other subjects '''<<BR>><<BR>> {{attachment:noise_copy.gif||height="126",width="273"}}

=== EEG noise covariance: Pre-stimulus baseline ===
 * In folder sub002/run_01_sss_notch, select all the imported the imported trials, right-click > '''Noise covariance > Compute from recordings''',  Time=[-500,-0.9]ms, '''EEG only''', '''Merge'''. {{attachment:noise_eeg.gif||height="280",width="650"}}
 * This computes the noise covariance only for EEG, and combines it with the existing MEG information. {{attachment:noise_display.gif||height="223",width="375"}}

=== BEM layers ===
We will compute a [[Tutorials/TutBem|BEM forward model]]  to estimate the brain sources from the EEG recordings. For this, we  need some layers defining the separation between the different  tissues of the head (scalp, inner skull, outer skull).

 * Go to the anatomy view (first button above the database explorer).
 * Right-click on the subject folder > '''Generate BEM surfaces''':  The number of vertices to use for each layer depends on your computing  power and the accuracy you expect. You can try for instance with '''1082 vertices '''(scalp) and '''642 vertices '''(outer skull and inner skull). <<BR>><<BR>> {{attachment:anatomy_bem.gif||height="232",width="603"}}

=== Forward model: EEG and MEG ===
 * Go back to the functional view (second button above the database explorer).
 * Model used: Overlapping spheres for MEG, OpenMEEG BEM for EEG ([[Tutorials/HeadModel|more information]]).
 * In folder sub002/run_01_sss_notch, right-click on the channel file > '''Compute head model'''.<<BR>>Keep all the default options . Expect this to take a while...<<BR>><<BR>> {{attachment:headmodel_compute.gif||height="313",width="643"}}

=== Inverse model: Minimum norm estimates ===
 * Right-click on the new head model > '''Compute sources [2016]''': '''MEG MAG + GRAD''' (default options) <<BR>><<BR>> {{attachment:sources_compute.gif||height="406",width="626"}}

 * Right-click on the new head model > '''Compute sources [2016]''': '''EEG''' (default bad channels).

 * At the end we have two inverse operators, that are shared for all the files of the run (single trials and averages). If we wanted to look at the run-level source averages, we could normalize the source maps with a Z-score wrt baseline. In this tutorial, we will first average across runs and normalize the subject-level averages. This will be done in the next tutorial (group analysis).<<BR>><<BR>> {{attachment:sources_files.gif||height="147",width="243"}}

== Time-frequency analysis ==
We will compute the time-frequency decomposition of each trial using Morlet wavelets, and average the power of the Morlet coefficients for each condition and each run separately. We will restrict the computation to the MEG magnetometers and the EEG channels to limit the computation time and disk usage.

 * In Process1, select the imported trials "Famous" for run#01.
 * Run process '''Frequency > Time-frequency (Morlet wavelets)''': Sensor types=MEG MAG,EEG <<BR>>Not normalized, Frequency='''Log(6:20:60)''', Measure=Power, '''Save average''' <<BR>><<BR>> {{attachment:tf_process.gif||height="479",width="628"}}
 * Double-click on the file to display it. In the Display tab, select the option "Hide edge effects" to exclude form the display all the values that could not be estimated in a reliable way. Let's extract only the good values from this file (-200ms to +900ms). <<BR>><<BR>> {{attachment:tf_display.gif||height="155",width="270"}}
 * In Process1, select the time-frequency file.
 * Run process '''Extract > Extract time''': Time window='''[-200, 900]ms''', '''Overwrite input files''' <<BR>><<BR>> {{attachment:tf_cut.gif||height="197",width="411"}}
 * Display the file again, observe that all the possibly bad values are gone. <<BR>><<BR>> {{attachment:tf_cutdisplay.gif||height="178",width="410"}}
 * You can display all the sensors at once (MEG MAG or EEG): right-click > 2D Layout (maps).<<BR>><<BR>> {{attachment:tf_2dlayout.gif||height="270",width="653"}}
 * Repeat these steps for other conditions (Scrambled and Unfamiliar) and the other runs (2-6). There is no way with this process to compute all the averages at once, as we did with the process "Average files". This will be easier to run from a script.
 * If we wanted to look at the run-level source averages, we could normalize these time-frequency maps.  In this tutorial, we will first average across runs and normalize the  subject-level averages. This will be done in the next tutorial (group  analysis).

== Scripting ==
We have now all the files we need for the group analysis ([[Tutorials/VisualGroup|next tutorial]]). We need to repeat the same operations for all the runs and all the subjects. Some of these steps are '''fully automatic''' and take a lot of time (filtering, computing the forward model), they should be executed from a script.

However, we recommend you always '''review manually''' some of the pre-processing steps (selection of the bad segments and bad channels, SSP/ICA components). Do not trust blindly any fully automated cleaning procedure.

For the strict reproducibility of this analysis, we provide a script that processes all the 19 subjects:  '''brainstorm3/toolbox/script/tutorial_visual_single_orig.m''' (execution time: 10-30 hours)<<BR>>Report for the first subject: [[http://neuroimage.usc.edu/bst/examples/report_TutorialVisual_sub001.html|report_TutorialVisual_sub001.html]]

<<HTML(<div style="border:1px solid black;  background-color:#EEEEFF; width:720px; height:500px; overflow:scroll;  padding:10px; font-family: Consolas,Menlo,Monaco,Lucida  Console,Liberation Mono,DejaVu Sans Mono,Bitstream Vera Sans  Mono,Courier New,monospace,sans-serif; font-size: 13px; white-space: pre;">)>><<EmbedContent("http://neuroimage.usc.edu/bst/viewcode.php?f=tutorial_visual_single_orig.m")>><<HTML(</div >)>>

<<BR>>You should note that this is not the result of a fully automated procedure. The bad channels were identified manually and are defined for each run in the script. The bad segments were detected automatically, confirmed manually for each run and saved in external files distributed with the Brainstorm package sample_group_freesurfer.zip (sample_group/brainstorm/bad_segments/*.mat).

All the process calls (bst_process) were generated automatically using with the script generator (menu '''Generate .m script''' in the pipeline editor). Everything else was added manually (loops, bad channels, file copies).

== Bad subjects ==
After evaluating the results for the 19 subjects, 3 subjects were excluded from the analysis:

 * '''sub001''': Error during the digitization of the EEG electrodes with the Polhemus (the same electrode was clicked twice). This impacts only the EEG, this subject could be used for MEG analysis. <<BR>><<BR>> {{attachment:bad_sub001.gif||height="158",width="274"}}
 * '''sub005''': Too many blinks or other eye movements. <<BR>> <<BR>> {{attachment:bad_sub005.gif||height="164",width="430"}}
 * '''sub016''': Too many blinks or other eye movements. <<BR>> <<BR>> {{attachment:bad_sub016.jpg||height="152",width="430"}}

Make sure you don't include these subjects in the group analysis.

<<EmbedContent("http://neuroimage.usc.edu/bst/get_prevnext.php?prev=&next=Tutorials/VisualGroupOrig")>>

<<EmbedContent(http://neuroimage.usc.edu/bst/get_feedback.php?Tutorials/VisualSingleOrig)>>