Digimouse: Data Acquisition


We created high quality images of PET, CT and cryosection slices markers readily apparent for later image registration that would not compromise the image acquisitions. We accomplished this by creating a wooden framework to which both the mouse and markers were attached. The markers consisted of small bore teflon tubing filled with black ink and F-18. The mouse was frozen by liquid nitrogen immersion after being sutured to the framework and was kept frozen during imaging of PET and CT by blowing cold nitrogen gas over the mouse. Following PET and CT imaging, the mouse and the framework was packed in CMC and froze it overnight at -20C. The next morning the cryostat was set up to cut the sections thoughout the entire mouse.


Current configuration does not allow embedding of the file sequence_new.gif because of its mimetype image/gif.: sequence_new.gif

The Raw Data of coronal PET, CT and Cryosection slices are:

pet.gif

ct.gif

cryosection.gif


Matrix size:
Voxel size:
Size:

PET
128 x 127 x 128
0.4 x 0.4 x 0.6 mm3
8MB

CT
512 x 942 x 512
0.1 x 0.1 x 0.1 mm3
900MB

Cryosection
1704 x 2560 x 418
38.8 x 38.8 x 50um3
600MB




Digimouse_Data_Acquisition (last edited 2012-02-12 07:25:04 by cpe-76-170-79-250)