SEEG Time-Frequency Fingerprint Analysis for Epileptogenic Zone Localization (under construction)

Authors: xxx.

This tutorial introduces some concepts that are specific to the management of intracranial, SEEG recordings in the Brainstorm environment, and explains how to compute maps of epileptogenicity from ictal and interictal recordings. It is based on a clinical case from the McGovern Medical School, University of Texas Health Science Center at Houston, Texas, USA.

Note that the operations used here are not detailed, the goal of this tutorial is not to introduce Brainstorm to new users. For in-depth explanations of the interface and theoretical foundations, please refer to the introduction tutorials.

NOT FOR CLINICAL USE:
The performance characteristics of the methods and software implementation presented in this tutorial have not been certified as medical devices and should be used for research purposes only.

Dataset description

License

This tutorial dataset (EEG, MRI and CT data) remains property of the McGovern Medical School, University of Texas Health Science Center at Houston, Texas, USA. Its use and transfer outside the Brainstorm tutorials, e.g. for research purposes, is prohibited without written consent. For questions, please contact Yash Shashank Vakilna, MS ( Yash.Shashank.Vakilna@uth.tmc.edu ).

Clinical description

The dataset was recorded at the Epilepsy Monitoring Unit at UTHealth Houston. It includes recordings for a patient who was a 25-year-old right-handed woman with drug-resistant epilepsy since age six and a prior right parietal opercular corticectomy at 15 presented with weekly focal aware seizures featuring a left-hand tingling aura and focal impaired awareness seizures with staring and pouting. In the Epilepsy Monitoring Unit (EMU) she had intermittent right parietal slowing and ten habitual seizures arising from C4-P4, and MRI revealed bilateral perisylvian polymicrogyria (PMG), pachygyria, right posterior temporal periventricular nodular heterotopia, and post-surgical changes. MEG localized discharges to the right superior parietal region adjacent to her previous resection, and SEEG implantation mapped two distinct onset patterns: low-voltage fast activity in right superior parietal PMG during focal aware seizures and repetitive spiking in posterior insular PMG during impaired awareness seizures. After multidisciplinary review, she underwent uncomplicated MR-guided laser interstitial thermal therapy targeting the right superior parietal and posterior insular PMG and remained seizure-free at one-year follow-up.

SEEG recordings

The depth electrodes used in this example dataset are PMT SEEG Depth Electrodes, with the following specifications:

• Diameter: 0.8 mm
• Contact length: 2 mm
• Insulator length: 1.5 mm
• Distance between the center of two contacts: 3.5 mm
• Between 8 and 16 contacts on each electrode

Files

tutorial_seizure_fingerprinting/

• anatomy/: Anatomy data

  • pre_T1.nii.gz: Raw pre-implantation T1 MRI (in NIfTI-1 format)

  • post_CT.nii.gz: Raw post-implantation CT scan (in NIfTI-1 format)

• recordings/: SEEG recordings

  • Baseline.edf: Raw SEEG recordings (in EDF format) for baseline

  • ictal_repetitive_spike.edf: Raw SEEG recordings (in EDF format) for seizure with Ictal repetitive spiking

  • interictal_spike.edf: Raw SEEG recordings (in EDF format) for interictal spike

  • LVFA_and_wave.edf: Raw SEEG recordings (in EDF format) for seizure onset with Low-voltage-fast-activity

• Subject01_electrodes_mm.tsv: Position of contacts localized using Brainstorm in world coordinates, relative to the post-implantation CT. The advanced section Electrode labeling and contact localization explains how it was done.

References

All details for this study can be found here: https://zenodo.org/records/14807262

Download and installation

• Requirements: You have already followed all the introduction tutorials and you have a working copy of Brainstorm installed on your computer.

• SPM: If you are running Brainstorm from the MATLAB environment, you need to have the SPM12 toolbox installed on your computer, as a Brainstorm plugin or a custom installation.
  With the stand-alone compiled version of Brainstorm: all the needed SPM scripts have been compiled and included in the executable. This is required for two purposes:

  • Coregistration between pre-implantation MRI and post-implantation CT volumes.

  • Use mask from SPM based tissue segmentation to remove extracranial regions from the CT.

• OpenMEEG: To compute the forward head model the OpenMEEG Brainstorm plugin is required.

• Download the dataset:

  • Go to the Download page of this website, and download the file: tutorial_seizure_fingerprinting.zip.

  • Unzip it in a folder that is not in any of the Brainstorm folders (program folder or database folder).

• Brainstorm:

  • Start Brainstorm

  • Select the menu File > Create new protocol. Name it "TutorialSeizureFingerprinting" and select the options:
    "No, use individual anatomy",
    "No, use one channel file per acquisition run".

Import the anatomy

Pre-implantation MRI

• Right-click on the TutorialSeizureFingerprinting folder > New subject > Subject01.
  Keep the default options you defined for the protocol.

• Switch to the Anatomy view of the protocol.

• Right-click on the subject node > Import MRI.
  Set the file format: MRI: NIfTI-1 (*.nii;*.nii.gz).
  Select: tutorial_seizure_fingerprinting/anatomy/pre_T1.nii.gz

• The MRI viewer opens automatically. Click on "Click here to compute MNI normalization", option "maff8". This method is embedded in Brainstorm and does not require the installation of SPM12. However, it requires the automatic download of the file SPM12 Tissue Probability Maps. If you do not have access to internet, see the instructions on the Installation page. It is based on an affine co-registration with the MNI ICBM152 template from the SPM software, described in the following article: Ashburner J, Friston KJ, Unified segmentation, NeuroImage 2005..
  
  

• Click on Save to close the MRI viewer. New node named pre_T1 is created.
  
  

Post-implantation CT

The pre-implantation MRI above will be used as the anatomical reference for this subject. We will now import a second scan done after the SEEG implantation, on which we can see the SEEG contacts. In this dataset, the post-implantation volume is a CT scan (contacts hypersignal appear in white).

• Right-click on the subject node > Import CT.
  Select: tutorial_seizure_fingerprinting/anatomy/post_CT.nii.gz

• Choose SPM for coregistration. See the section Volume coregistration for more details on this option.
  Choose Yes for reslicing so that the CT voxel dimensions match those of the MRI.
  
  

• Choose SPM for skull stripping to remove any non-brain tissues as we need just the electrodes inside the brain. See the section Skull Stripping for more details on this option.
  
  

• The MRI viewer opens automatically, showing the post-implantation CT volume as a colored layer on top of the previous volume. Adjust the transparency and amplitude threshold of this layer in the section Data options of the Surface tab, adjust its colormap with the popup menu of the figure. Use this display to validate that the coregistration of the two volume is correct, all the parts of the head must align well.
  
  

• New node named post_CT_spm_reslice_masked_spm is created. The postfix _spm_reslice_masked_spm indicates the different processes it has gone through. To see the history of the processes for this file, right click on post_CT_spm_reslice_masked_spm > File > View file history.
  
  

• To just view the CT File, right click on post_CT_spm_reslice_masked_spm > Display > MRI Viewer.
  
  

Generate default surfaces using CAT12

We recommend generating cortical surfaces with CAT12, especially if you are interested in a realistic representation of the patient's cortical folding in 3D. Follow the CAT12 tutorial to generate the surfaces as under.

These surfaces will be used later, in the computation of the epileptogenicity maps. Read the section Importing realistic surfaces for information on how to use realistic surfaces from BrainVISA or FreeSurfer.

Electrode labeling and contact localization

If you do not have any recordings in the database, Brainstorm allows to creation and annotation of intracranial electrodes and contacts. Users can also then export these as a text file with all the positions that can be used in Brainstorm or any other program.

Generate isoSurface

This creates a thresholded mesh from the CT by separating the contacts out from rest of the CT. This aids the user towards localization of the electrodes and its contacts more accurately.

• The first thing required is to define a good threshold (which we call isoValue) that will separate out the contacts from the rest of the CT. Just open the CT file (by double clicking on it), click on the MIP: Functional in the MRI Viewer, use the Data Options > Amplitude slider in the Surface tab to browse through the threshold, and once you are satisfied with getting a good separation of the contacts, hover on the slider to get the desired value and keep a note of it (to the nearest integer). In this case the value is 2276.
  
  

• Right click on post_CT_spm_reslice_masked_spm> CT segmentation > Generate SEEG/ECoG isosurface.
  
  

• This will bring the Generate isosurface window. This window shows 4 values: the Background level, White level, Max Intensity and the suggested Set isoValue, all of them given in the Hounsfield Unit (HU) scale. The first 3 values are calculated automatically from the histogram of the CT and are displayed for reference. The editable isoValue field shows an estimated best guess based on mean of White Level and Max Intensity. Since we got a better value in step-1 above, set the value to 2276 and press OK.
  
  

• An isosurface is generated showing the contact as blobs overlayed on the 3D MRI slices. The Thresh slider under Surface options can be further used to fine tune and regenerate mesh with different isoValues.
  
  

Start implantation

• Right click on Subject01, and choose SEEG/ECOG implantation.

• The SEEG/ECOG implantation menu pops to choose which modalities you want to use for doing your implantation with. Choose MRI+CT+IsoSurf. This takes you to the functional tab. Subject01 > Implantation > SEEG/ECOG (0) channel gets created. An MRI Viewer loads up with the CT overlayed on the MRI. A 3D figure loads up with isoSurface and 3D MRI. Panel iEEG loads up in the panel viewer. Move around the 3D slices to get a clear view of the contacts so that they can be clicked on. See the section SEEG/ECOG Implantation Menu for more details on this option.
  
  
  
  

Create electrodes and plot contacts manually

• After performing steps above, we will have the Panel iEEG along with the MRI Viewer and 3D figure open. We will be working on the electrode marked below.
  
  

• On Panel iEEG Click on the + (Add new electrode). This opens up the Add electrode window. Enter anything under Electrode label based on your convention. For this e.g. enter POP and press OK.
  
  

• This creates an electrode POP in selected state, and at the bottom the Electrode configuration section of the panel becomes active. Select the following:

  • Type: SEEG

  • Model: Choose PMT 2102-16-093/2102-16-103 from the drop down list. This will automatically set the other parameters in the section. More details for these parameters can be found in the advanced section here.
    
    

• Set tip: Choose the location of the electrode's contact that is deepest in the brain. While we can use the MRI Viewer to set this location (as mentioned here), but to get a more accurate location we will leverage the 3D isosurface as described below.

  • As seen in the first figure in this section, the tip contact blob is not well separated form its adjacent contact. As mentioned in the Generate Isosurface section, switch to Surface tab, use the Thresh slider to adjust the isovalue to approximately 3100 and a readjusted isosurface will be generated that separates out the tip area contacts.
    
    

  • Switch back to the iEEG tab, click the button (shortcut: Ctrl+P) to activate surface point selection mode.

  • Click on the button to activate selecting centroid of the candidate contact blob in the isosurface.

  • Choose the tip contact in the readjusted 3D isosurface and this should plot a yellow crosshair marker point on the center of the blob and also update the MRI Viewer's crosshair. At the bottom in click Set tip and the button turns green indicating that the tip has been set. This point in 3D is at the centroid of the blob which gives a more accurate location of the contact compared to the same if chosen from the MRI.
    
    

• Set skull entry: Choose the point in the 3D isosurface which is going to be set as the skull entry. This point can be any of the contacts other than the tip preferably closer to the skull so that we get an orientation of the electrode.

  • This should plot a yellow crosshair marker point on the blob and also update the MRI Viewer's crosshair.
    
    

  • At the bottom of iEEG tab, click Set skull entry and the button turns green indicating that the entry point has been set. All the figures get updated with the an electrode based on the above configuration. The properties used for setting the position of the contacts are the contact spacing, the tip of the electrode and the entry point in the skull. Contact #i is placed along the electrode at (i-1)*contact_spacing millimeters from the tip of the electrode.
    
    

• Click on Contacts > Save Modifications to update the channel information in the database.
  
  

• Repeat the steps for creating implantations for all the other electrodes one by one and save the modifications. Each time you can see the channel file SEEG/ECOG being updated.

• When you are done with everything, close all the figures and a prompt appears asking to save the changes. Just click Yes for everything.

Export the contacts position

• Right click on the Implantation > SEEG/ECOG channel file, click File > Export to file, choose type as EEG: BIDS electrodes.tsv, subject space mm (*.tsv), set file name as Subject_electrodes_mm.tsv click Save, set the export reference MRI as post_CT_spm_reslice_masked_spm (as the implantation was done on it) and press OK.
  
  

• This exports the positions in the file in world coordinates.

• The tutorial dataset already contains this implantation exported locations (tutorial_seizure_fingerprinting/Subject01_electrodes_mm.tsv) which will be used to link to the recordings in the section below.

Access the recordings

Link the recordings

• Switch to the "functional data" view (2nd button, on top of the database explorer).

• Right-click on the subject folder > Review raw file:

  • Select the file format: EEG: EDF/EDF+

  • Select all the recordings: tutorial_seizure_fingerprinting/recordings/*.edf

• The new files Link to raw file let you access directly the contents of the original SEEG files. The menu Review raw file does not actually copy any data to the database. More details.
  
  

Import the contacts positions

In order to generate epileptogenicity maps, we need accurate 3D positions for the contacts of the depth electrodes. Placing the contacts requires a good understanding of the implantation scheme reported by the neurosurgeon, and some skills in reading MRI scans. To make this tutorial easier to reproduce and follow, we distribute the positions of the contacts (that were localized as per the section above) saved in a .tsv file.

• The channel file "EDF channels" contain the name of the channels, but not their positions. We need to import or edit separately the positions of the SEEG contacts.

• Click on the [+] next to the four folders, select all the channel files simultaneously.

• Right-click one channel file > Add EEG positions > Import from file

• Select format: EEG: BIDS electrodes.tsv, subject space mm (*.tsv)

• Select file: tutorial_seizure_fingerprinting/Subject01_electrodes_mm.tsv
  
  

• Select a scaling factor: 1
  The positions in this text file are in millimeters, the expected unit for this file format is also millimeters, there is no adjustment to make here.
  
  

• For the reference MRI, select the reference MRI as post_CT_spm_reslice_masked_spm since the Subject01_electrodes_mm.tsv file consists of contact positions which were localized on it within Brainstorm.
  
  

• At the end, you get a report indicating how many channels from the SEEG recordings were attributed a new 3D position. The channels are matched by name: the position file you import must include the labels of the channels and they must be named exactly in the the same way as in your recordings.
  
  

• For EDF Channels under LVFA_and_wave and interictal_spike, there were 12 channels not found. The type of the channels for which a position was not found is set to EEG_NO_LOC, in order to ignore them when processing the SEEG data. In this example dataset, the channels numbered 245 to 255 and 262 are not found as they are not SEEG contacts.

• For all the EDF Channels, mark the channel MPS16 as SEEG_NO_LOC because if not marked, when we compute the forward head model using BEM surfaces it says that MPS16 lies outside the BEM layer. Marking it excludes it from any future computations.

• You should always validate that the type of all the channels has been detected correctly. Check also that the names are correct and using the same convention for all the contact of a given electrode. These are entered manually, and typing errors are frequent. Right-click on a channel file > Edit channel file.
  
  

• To edit one channel, double-click on the text to modify. To edit multiple channels, select them all and right-click > Set channel group/type. The column Group corresponds to the name of the depth electrode. It is detected based on the channel type and name. If you include as SEEG a channel that detected as something else or rename a channel, you would need to manually update the channel name.

• If you don't have the positions for the SEEG contacts, or if they don't look correctly aligned, see the section Edit the contacts positions on how to tackle it.

Display the depth electrodes

3D figures

• Right-click on the channel file > Display sensors and explore all the available options.
  
  

• You can render the SEEG depth electrodes in 3D together with the subject anatomy: surfaces, pre- or post-implantation volumes. You can add more anatomy elements to the figure with the button Add a surface ( ) at the top-right of the Surface tab. For more help: Display the anatomy.
  
  

• Click on a contact to select it, right-click on it to get its name.
  
  

MRI Viewer

• You can also display the contacts in the MRI viewer, on top of the the pre- or post-implantation volumes. By default, the electrode is displayed in a slice if there is a SEEG contact associated to it in the slice.
  
  

• To display all the electrodes, select the option "MIP: Functional". For a glass-brain view, select at the same time the option "MIP: Anatomy".
  
  

• Zoom in/out with the buttons or the associated shortcuts (Ctrl+Scroll or +/-) and explore the volume with (Shift+)x/y/z. Additional display options are available in the popup menu for this figure. All the shortcuts are listed in this tutorial.

• You can edit the position of a contact directly clicking on its dot in one of the slice and moving it around. To do it you need to open the MRI Viewer in Edit mode by right clicking on the channel file > MRI registration > Edit ... (MRI Viewer:...). Modifications are not saved immediately and can be cancelled when you close the window. Alternatively, you can right-click on the figure > Set electrode position.
  
  

Panel iEEG

• When opening SEEG/ECOG recordings, the panel iEEG is added to the Brainstorm window. You can use it to edit the display properties of the depth electrodes. More details about the iEEG Panel can be found in the Epileptogenicity tutorial.
  
  

Display SEEG recordings

To know more about ways to display the SEEG recordings in Brainstorm refer to the Epileptogenicity tutorial.

Review recordings

Power spectrum

We recommend you always start your data analysis with a spectral evaluation of the recordings. This is described in more details in the tutorials Power spectrum, EEG and epilepsy and Epileptogenicity.

• In Process1, select all the continuous files (Link to raw files or folders).
  
  

• Double-click on the PSD files to display them. Some channels have noise levels that are obviously higher than the others and should therefore be considered as suspicious.

  • In the Baseline PSD, peaks related with the power lines: 60Hz, 180Hz, 300Hz, 420Hz, 540Hz, 660Hz, 780Hz and 900Hz can be seen. Applying the notch filter process can be used to remove these frequencies.

  • The interictal spike, ictal repetitive spike and LVFA and wave are subsets of the Baseline recordings which have all the 60Hz+harmonics removed and it can clearly be seen from their respective PSDs in the figure above.

Add annotations

We need to annotate seizure onset event for the ictal and LVFA and wave recordings and spike event for interictal recording. There are events already available in recordings, that were marked for clinical use, to jump quickly to the page of interest. More details can be found in the tutorial Event Markers.

• For LVFA and wave, we will create a new event sEEG Onset at around 15s (center of the 30s recording extract).
  
  

• For ictal repetitive spike, we will create a new event sEEG Onset at around 15s (center of the 30s recording extract).
  
  

• For interictal spike, we will create a new event Interictal spike at around 5s (center of the 10s recording extract).
  
  

Import epochs of interest

At this point of the analysis, we are still looking at the original files, no SEEG data was copied to the database. The montages are saved in the Brainstorm preferences, the new events are saved in the links of the database.

We are now going to import the three segment of recordings i.e. LVFA and wave , ictal repetitive spike and interictal spike which are a subset of the Baseline recording.

Import in database

• Right click on LVFA_and_wave > Link to raw file > Import in database.

  • Time window: All (0 to 30.0495)
  • Split in time blocks: Disabled

  • Use events: Enabled, select sEEG Onset

  • Epoch time: [-15000, +15000] ms (imports -15s to 15s around the event sEEG Onset)

  • Remove DC offset: Enabled. More details can be found here.

  • Resample: Disabled
  • Create a separate folder for each event type: Disabled

  • Click Import.
    
    

• Repeat the same steps above for importing ictal repetitive spike into the database.

• Repeat the same steps above for importing interictal spike into the database with the modification in the following options:

  • Time window: All (0 to 10.0495)

  • Use events: Enabled, select Interictal spike

  • Epoch time: [-5000, +5000] ms (imports -5s to 5s around the event Interictal spike)

• At the end, you should have three new folders LVFA and wave , ictal repetitive spike and interictal spike, the same name as the original raw files, but without the tag RAW on top. These new folders contain copies of the SEEG recordings, if you delete these folders from the database explorer, you lose the recordings they contain.

• The imported epochs are saved with a new timing: for LVFA and wave and ictal repetitive spike, the reference time t=0s is now the event sEEG Onset and for Interictal spike, the reference time t=0s is now the event Interictal spike, which has been removed from the list. You can still see the other marker timings adjusted accordingly.
  
  

Bipolar montage

We will run the rest of the analysis using a bipolar montage (bipolar-2). The montage selected in the Record tab is for visualization only, most processes ignore this selection and work only on the original common-reference data. To force all the processes to work on the bipolar values, we need to explicitly apply the montage to the recordings. More details can be found in tutorials Montage editor and Epileptogenicity.

• In Process1, drag and drop all the imported recordings (either the folders or the files).

• Run the process Standardize > Apply montage

  • Montage name: Subject01: SEEG (bipolar 2)[tmp]

  • Create new folders: Enabled
    
    

• This process rewrites the channel files and data files in the selected folders. The position associated with a bipolar channel in the channel file is the middle of the segment between the two contacts (e.g. AH1-AH2 is placed half-way between AH1 and AH2).
  
  

Head modeling

The forward models depend on the subject's anatomy, including head size and geometry, tissue conductivity, the computational method, and sensor characteristics. In this section, we will use the Boundary Element Method (BEM) approach available in Brainstorm for constructing the head model for sEEG. For more details on head modeling please refer to the tutorial Head modeling. Follow the tutorial Realistic head model: BEM with OpenMEEG to generate BEM head surfaces from the MRI.

• This will generate three surfaces head, outer skull and inner skull estimated from the head mask and the cortex obtained from the segmentation. The BEM surfaces will be used for the BEM forward computation later in the tutorial.
  
  

Modeling interictal spikes using Min-Norm Imaging

Modeling ictal wave using Min-Norm Imaging

Modeling ictal onset with Low Voltage Fast Activity (LVFA) using fingerprint analysis (Sensor Space)

Modeling ictal onset with LVFA using fingerprint analysis (Source Space)

Modeling ictal onset with repetitive spiking (Sensor and Source Space)

SEEG/ECOG Implantation Menu

While starting manual implantation on an volume by right click on subject node > SEEG/ECOG implantation, the menu below pops up allowing the user to choose which modality or modalities to proceed with.

• MRI: MRI viewer loads up with MRI volume only.

• CT: MRI viewer loads up with CT volume only.

• MRI+CT: MRI viewer loads up with the CT overlayed on the MRI.

• MRI+CT+IsoSurf: MRI viewer loads up with the CT overlayed on the MRI. 3D figure loads up with the IsoSurface and 3D MRI slices.

• Cancel: Abort the process.

Skull Stripping

Skull stripping is done on the MRI volume to get a binary volumetric mask of just the brain region. On applying this mask to volumes like CT, it helps in removing any non-brain tissues like skull, scalp, fat, and any other head tissues from the CT volume. In this tutorial, we have a CT scan of a subject with intracranial electrodes, so on applying this mask not only removes the non-brain tissues but also the hanging extracranial wires. Brainstorm has the following menu options for skull stripping:

• SPM: Uses SPM toolbox's Tissue Segmentation.

• BrainSuite: Uses BrainSuite's Brain Surface Extractor. Make sure you have the latest version of BrainSuite installed. Follow steps as per the BrainSuite for Brainstorm tutorial.

• Skip: Proceed without skull stripping.

Edit the contacts positions

The trajectory of electrode while implantation may not always follow a straight line as there could be bending introduced when the neurosurgeon inserts the electrode. In such cases we need to move these contacts to more appropriate positions.

• For this section we will consider one such electrode from our dataset above which is marked below. Switch to anatomy tab, open the isoSurface file under Subject01 and using the Thresh slider in the Surface tab set the isoValue to approximately 2280.
  
  

• This is a complex case where:
  • The tip is not well separated from its adjacent contact.
  • One of the contacts in the middle can be seen joined to another electrode's contact.

• Play with the Thresh slider under Surface tab to get a good threshold that separates them out.

• As per the sections Start implantation and Create electrodes and plot contacts manually above, create an SEEG electrode named SPM with model PMT 2102-16-091/2102-16-101. Switch to Surface tab first and change the isoSurface threshold to approximately 2680 to get a clean tip for marking and then switch back to iEEG tab to do the implantation.

• Set the isosurface threshold back to approximately 2280 for clear view of the 3D blobs. Select the 3D figure and toggle between the electrode ( ) and sphere ( ) modes to get the figures as under using buttons in iEEG panel. Keep it in the sphere mode for better visibility. The contacts definitely need to be corrected and moved to their right positions.
  
  

• Display the contact labels in 3D figure by right clicking Channels > Display labels. It can be seen that the contacts SPM2-SPM16 are incorrect. Let us first correct contact SPM2.
  
  

• Switch to Surface tab, set the isosurface threshold back to approximately 2680 for separating out the tip and its adjacent contact blob (SPM2). Since the isosurface blobs are the correct ones, we can use them as ground truth guide to correct the contacts. Switch to iEEG tab, click the button (shortcut: Ctrl+P; to turn on surface point selection), click the button (to turn on centroid selection in surface) and click the correct 3D blob. That places a yellow crosshair on the blob and updates the MRI Viewer crosshair as well.
  
  

• Without clicking anywhere else, move your cursor to the MRI Viewer, right click and go to Electrodes > Set electrode position (or press Ctrl+S). From the Set electrode position window choose SPM2 and press OK. This will update the position of SPM2 only in the MRI Viewer.
  
  

• Click on Save and it closes all the windows and updates the channel file and number of channels in the database explorer.

• Right click on Subject01 > SEEG/ECOG implantation. Click Continue for working on the existing implantation. Choose MRI+CT+IsoSurf to show all the figures. Switch to Surface tab, set the isosurface threshold back to approximately 2280 for clear view of the 3D blobs. Select the 3D figure and in the Panel iEEG, choose the sphere ( ) mode for display. You will now see the updated contact in the 3D figure as well.
  
  

• Follow the above steps to update all the remaining contacts as required. Unfortunately, Brainstorm currently does not have a way to handle curved electrodes display yet: if you move the contacts, the electrode remains represented as a straight line. This is a display issue only, which has no impact on any computation, but we hope to improve it at some point.
  
  

• We have a functionality called line fitting that can help better view the trajectory in the 3D figure with sphere ( ) display mode. Choose the electrode and click Contacts > Show/Hide line fit through contacts.
  
  

• Additionally, there are features like Add contact and Remove contact that can be used to edit the contacts as well.

Additional Documentation

Forum discussions

• Updating SEEG coordinates

Related tutorials

• Epileptogenicity

• ECoG/sEEG tutorial