Authors: Francois Tadel, Marcel Heers.
This tutorial introduces some concepts that are specific to the management of ECoG/sEEG recordings in the Brainstorm environment. It is based on a clinical case from the Epilepsy Center at the University Hospital of Freiburg, Germany.
Note that the operations used here are not detailed, the goal of this tutorial is not to introduce Brainstorm to new users. For in-depth explanations of the interface and theoretical foundations, please refer to the introduction tutorials.
NOT FOR CLINICAL USE:
The performance characteristics of the methods and software implentation presented in this tutorial have not been certified as medical devices and should be used for research purposes only.
- Dataset description
- Download and installation
- Import the anatomy
- Access the recordings
- Edit the contacts positions
- Alternative: Read BIDS electrodes.tsv
- Display the recordings
- Import epochs of interest
- Anatomical labelling
- Additional documentation
This tutorial dataset (EEG and MRI data) remains proprietary of the Epilepsy Centre, University Hospital Freiburg, Germany. Its use and transfer outside the Brainstorm tutorial, e.g. for research purposes, is prohibited without written consent from the Epilepsy Centre in Freiburg. For questions please contact A. Schulze-Bonhage, MD, PhD: firstname.lastname@example.org
We would like to thank the patient for providing his dataset for this tutorial, the clinical team of the Epilepsy Center Freiburg for the aquisition of the dataset and Verena Schulte for her help in preparing the dataset for the tutorial.
The patient is a 38yr male who suffered from drug resistant focal epilepsy since his 13th year of age with two different types of focal aware seizures that rarely evolved at nighttime to bilateral tonic-clonic seizures. Seizure type I consisted of daily aware focal seizures with myoclonic or clonic jerks of the right leg that occasionally led to falls. Type II consisted of formication paraesthesias that propagated from the right calf to the hip region. It could be provoked by stress or hot weather and occurred 2-3 times a week. In the presurgical 3T epilepsy MRI no structural abnormality was detected.
After non-invasive telemetry intracranial recordings were performed. It was decided to implant a 64 channels Ad-Tech subdural grid with 8x8 contacts (stainless steel, 10mm between centers of contacts, 2.3mm diameter exposure) over the left fronto-centro-parietal convexity covering the central region. The most inferior lateral row of contacts of the grid electrode is named A and the row of contacts next to the midline is named H. The contacts of the grid have numbers between 1-8 with the most posterior contacts of each row starting with number 1.
Additionally, 3 interhemispheric Ad-Tech strip electrodes with 4 contacts each named from posterior to anterior with IHA being the most posterior followed by IHB and IHC being the most anterior strip electrode. The numbering convention is that the deepest contact is named contact "1": and the most superficial contact of each electrode has the highest number.
In addition, 2 Ad-Tech sEEG electrodes were implanted in the right parietal lobe facing to the insula (TA & TB, distance between centers of contacts: 10mm, diameter: 0.86mm). Both sEEG electrodes have 10 electrode contacts each. The contacts are counted from anterior to posterior starting with contact number 1 at the tip of each sEEG electrode.
The figure illustrating the electrode positions was created as reported by Kovalev et al. 2005.
The EEG data distributed here was recorded at 1024Hz, using a Neurofile NT digital video-EEG system with 128 channels and a 16-bit A/D converter. The signal was filtered in the recording system with a high-pass filter with a time constant of 1 second (cut-off frequency ~ 0.16Hz) and a low-pass filter with a cut-off frequency of 344 Hz.
Repetitive sharp waves and interictal fast epileptic activity in the gamma frequency band (FEA; for advanced spectral analysis of FEA see also (Heers et al., 2018)) was almost continuously recorded in contacts G5-6 extending towards H5-6 or F5-6 and less frequently expanding to H3-4 & 7, IHA1-3, IHB1-3
- Seizure onset pattern: Repetitive gamma bursts interrupted by sharp slow waves in G5-6, H3-4 and IHA1-3
- Semiology: Myoclonic and clonic movements of the right leg intermingled with not clearly localizing habitual formication paraesthesias of the right leg
- Stimulation of contacts G5: habitual myoclonic seizures
- Stimulation of contact G6: habitual paraesthesias of the right leg
- Detailed function mapping was performed, which is not reported in detail here.
Resection, histopathology and postsurgical outcome:
- Resection of the posterior part of the superior frontal gyrus anterior of the precentral gyrus including the cingulate gyrus was performed. Because there was overlap between the primary motor cortex (confirmed by functional mapping) at contact H3 position H3, which was also involved in the seizure onset zone this brain area was spared and only multiple subpial transections were added here.
- Histological examination revealed focal cortical dysplasia type Ib (Palmini et al., 2004). After surgery the patient developed a severe SMA syndrome with diminished activity, problems in movement initiation and speech production from which he recovered completely after rehabilitation.
- After surgery the patient remained completely seizure free (Engel IA outcome, follow up 10 years).
Heers M, Helias M, Hedrich T, Dümpelmann M, Schulze-Bonhage A, Ball T (2018): Spectral bandwidth of interictal fast epileptic activity characterizes the seizure onset zone. NeuroImage Clin 17.
Kovalev D, Spreer J, Honegger J, Zentner J, Schulze‐Bonhage A, Huppertz HJ (2005): Rapid and fully automated visualization of subdural electrodes in the presurgical evaluation of epilepsy patients. AJNR Am J Neuroradiol 26:1078–1083.
Palmini A, Najm I, Avanzini G, Babb T, Guerrini R, Foldvary-Schaefer N, Jackson G, Luders HO, Prayson R, Spreafico R, Vinters H V (2004): Terminology and classification of the cortical dysplasias. Neurology 62:S2-8.
The dataset we distribute with this tutorial follows the Brain Imaging Data Structure (BIDS) standard for neuroimaging data organization. This specification was first established for MRI and fMRI (Gorgolewski, 2016) and then refined with an extension dedicated to iEEG (Holdgraf, 2019). The files that will be imported in this tutorial are the following:
derivatives/: Everything that cannot be considered as raw data
freesurfer/sub-ecog01_ses-preimp/: Result of the FreeSurfer 6 segmentation for all the subject ecog01 (pre-implantation)
sub-ecog01/: Raw data for subject ecog01
ses-preimp/: Imaging exams performed before the implantation of the ECoG/sEEG.
anat/sub-ecog01_ses-preimp_T1w.nii.gz: T1-weighted MRI pre-implantation
ses-postimp/: Exams performed with the sEEG/ECoG devices implanted.
anat/sub-ecog01_ses-postimp_T1w.nii.gz: T1-weighted MRI post-implantation
ieeg/..._task-seizure_run-01_ieeg.eeg: 1 hour ECoG+sEEG recordings with 3 seizures (saved using the BrainVision file format, with the header files .vhdr and .vmrk)
ieeg/..._space-IXI549Space_electrodes.tsv: Position of the contacts in MNI space
ieeg/..._space-other_electrodes.tsv: Position of the contacts in world coordinates
All the anatomical images have been de-identified with mri_deface from FreeSurfer 6.
Download and installation
Requirements: You have already followed all the introduction tutorials and you have a working copy of Brainstorm installed on your computer.
SPM: The coregistration between pre- and post-implantation MRI volumes requires the SPM12 toolbox. If you are running Brainstorm from a Matlab installation, SPM can be installed or configured as a plugin: menu Plugins > SPM12 > Install. If you are using the stand-alone compiled version of Brainstorm: all the needed SPM scripts have been compiled and included in this executable.
Download the dataset:
Go to the Download page of this website, and get the file: sample_ecog.zip
- Unzip it outside of any of the Brainstorm folders (program folder or database folder)
- Start Brainstorm (Matlab scripts or stand-alone version).
Select the menu File > Create new protocol. Name it "TutorialEcog" and select:
"No, use individual anatomy",
"No, use one channel file per acquisition run".
Import the anatomy
The entire dataset, anatomy and recordings, is organized following the BIDS specification. It could be loaded automatically with the menu Import BIDS dataset, as illustrated in the Resting/OMEGA tutorial. However, in this tutorial we will detail the step-by-step instructions to load the anatomy and ECoG recordings manually.
The pre-implantation T1 MRI is available as a .nii file (sample_ecog/sub-ecog01/ses-postimp/anat). This volume was processed with FreeSurfer 6, and the output segmentation is available in the derivatives folder (sample_ecog/derivatives/freesurfer/sub-ecog01/ses-preimp). We don't need to import both to the Brainstorm database: in order to minimize the number of operations, we will import only the FreeSurfer folder.
- Switch to the "anatomy" view of the protocol.
Right-click on the TutorialEcog folder > New subject > ecog01
- Keep the default options you set for the protocol
Right-click on the subject node > Import anatomy folder (auto): More information
Set the file format: FreeSurfer + Volume atlases
Select the folder: sample_ecog/derivatives/freesurfer/sub-ecog01_ses-preimp
At the end, make sure the file "cortex_15000V" is selected (downsampled pial surface), otherwise double-click to select it as the default cortex surface.
When processing your own datasets: Instead of using FreeSurfer, you can decide to run the segmentation of the pre-implantation MRI directly from Brainstorm, using CAT12.
For help with using the volume atlases, refer to the introduction tutorial Explore the anatomy.
- The pre-implantation MRI will be used as the anatomical reference for this subject. We will now import a second scan done after the sEEG/ECoG implantation, on which we can see the recording contacts. In this dataset, the post-implantation volume is another T1 MRI scan (contacts hyposignal appears in black), but it is more commonly a CT scan (contacts hypersignal appears in white).
Right-click on the subject node > Import MRI:
Do you want to apply the transformation to the MRI file? YES
This will reorient the MRI in Brainstorm's standard orientation, so you can see the coronal/sagittal/axial views correctly oriented.
How to register the new volume? SPM
For more information about these options, see the section Volume coregistration. Note that you need to have the SPM12 toolbox installed on your computer and added to your Matlab path for using this registration method.
Reslice the volume? NO
This operation rewrites the volume with the orientation and resolution of the pre-implantation MRI, so that the two volumes can be overlaid in the MRI viewer. In this tutorial, we are going to place the ECoG/sEEG contacts manually on this post-implantation volume. For a better image quality, it is better to do it using the non-resliced volume. We do want the resliced volume, at least to check that pre- and post-implantation MRI volumes are correctly registered, but we can compute the resliced volume separately.
Right-click on the post-implantation volume > Register with default MRI > Reslice / world coordinates.
Right-click on the resliced post-implantation volume > Display > Overlay on default MRI
The MRI viewer opens, showing the post-implantation volume as a colored layer on top of the previous volume. Adjust the transparency and amplitude threshold of this layer in the section "Data options" of the Surface tab, adjust its colormap with the popup menu of the figure. Use this display to validate that the coregistration of the two volume is correct, all the parts of the head must align well.
Generate skull surfaces
Right-click on the subject folder > Generate BEM surfaces > Brainstorm. Keep the default options.
When the computation is done, double-click on the the original head surface ("head mask") to select it as the default instead of the new BEM version, which is not estimated well because the volume was defaced.
Access the recordings
Link the recordings
- Switch to the "functional data" view (2nd button, on top of the database explorer).
Right-click on the subject folder > Review raw file:
Select the file format: "ECOG: Deltamed/Micromed/..."
Select the recordings: sample_ecog/sub-ecog01/ses-postimp/ieeg/..._ieeg.eeg
The new "Link to raw file" entry lets you access directly the contents of the original file. The menu "Review raw file" does not actually copy any data to the database. More information.
Edit the channel types
At the previous step, we explicitly defined that we were importing ECoG recordings. However, this file contains both sEEG and ECoG recordings, therefore we need to modify manually the type of some channels.
Right-click on the channel file > Edit channel file.
Select all the TA and TB channels, right-click > Set channel type: enter SEEG.
Edit the contacts positions
For 3D mapping and to generate epileptogenicity maps, we need accurate 3D positions for the contacts of the grids and sEEG electrodes. Placing the contacts requires a good understanding of the implantation scheme reported by the neurosurgeon, and some skills in reading MRI scans. This tutorial will now explain how to do this manual marking with the MRI Viewer from the post-implantation MRI, which shows clear hyposignal artifacts around the ECoG and sEEG contacts. We prefer using the non-resliced volume because it offers a clearer view of these artifacts.
If you have no interest in this or if you want to skip faster to the analysis of the signals, you can load the 3D positions from the BIDS dataset: read the advanced section Read BIDS electrodes.tsv.
ECoG grid: G
Right-click on the channel file > MRI registration > Edit (MRI Viewer: sub-ecog01_ses-postimp_T1w_spm).
Adjust the brightness and contrast of the colormap in order to see the contacts artifacts correctly: right-click on the figure and move your mouse up/down, or left-click on the colormap and move your mouse up/down (brightness) or left/right (contrast), or right-click on the figure > Colormap:Anatomy.
In the iEEG tab, select "G", and select the information we know about this grid:
Number of contacts: 8 8 (8x8 grid, for a total of 64 contacts)
Contact spacing: 10 mm
Contact height: 1 mm
(the grid is thinner, but giving it some thickness makes the figures easier to read)
Contact diameter: 4 mm
(Ad-Tech contacts have 4mm diameters, with a 2.3mm exposure)
- Wire width: Keep the default value, or adjust it later to your taste
- ECoG grids can be defined by pointing successively at their 4 corners. In this example, the contacts of the grid G are labelled sequentially: A1 A2 .. A8 B1 B2 .. B8 .. .. H1 H2 .. H8. You should point at the 4 corners starting by the first two ones, then finish rotating in the same direction: A1, A8, H8, H1.
Navigate in the volume and position the cursor at the center of the artifact corresponding to contact G_A1 (look at the implantation schemes above, it is the more frontal and mesial in the grid), then click on button [#1] in the iEEG tab.
If you can't find this point, you could enter manually the coordinates of the point: click on the button "Set current coordinates" at the bottom of the MRI Viewer window, and copy the MRI coordinates from the screen capture below.
Zoom in/out with the buttons or the associated shortcuts (Ctrl+Scroll or +/-) and explore the volume with (Shift+)x/y/z. Additional display options are available in the popup menu for this figure. All the shortcuts are listed in this tutorial.
Place the cursor at contact G_A8 and click on button [#2].
Place the cursor at contact G_H8 and click on button [#3].
Place the cursor at contact G_H1 and click on button [#4].
After placing the last corner, it should start computing for a while. The positions of the 64 contacts are distributed in the polygon you defined, and then adjusted to the shape of the brain. To get the figure below, with all the contacts projected on all slices, select the checkbox "MIP: Functional" in the MRI Viewer.
In this window, the contacts can be moved by simple drag-and-drop of the dots representing them. If you move them by mistake, there is unfortunately no undo button. But you can cancel your modifications in two ways: close the figure and discard the modifications, or select "G" in the iEEG tab and then use the menu Contacts > Use default positions, to recompute the initial positions from the 4 four corners of the ECoG grid.
In the same menu Contacts, you can find additional projection options: Project on inner skull and Project on cortex. All these menus work well only when skull surfaces are available for the patient (see the section above that explains how to use the menu Generate BEM surfaces).
From time to time, you can click on the menu Contacts > Save modifications in the iEEG tab. This would prevent you from losing your work if anything unexpected happens.
ECoG strips: IHA, IHB, IHC
In the iEEG tab, select strip IHA and edit the options:
Type: ECOG-mid (for strips or grids that are not in aligned with the skull surface)
Number of contacts: 4
Contact spacing: 10 mm
Contact height: 1 mm
Contact diameter: 4 mm
- ECoG strips are defined only with two points, the first and the last contact. The intermediate contacts are uniformly distributed between the first and last point. In the convention used here, the first contact is always the deepest one.
If you want to hide the contacts of previously edited devices: select the item in the list at the top of the iEEG tab, then click on the button Show/hide selected electrodes just above the list.
Place the cursor on IHA1 (the deepest contact on the most posterior strip) and click [#1]. To jump directly to the coordinates of this point: click on the button Set current coordinates at the bottom of the MRI viewer, and report the MRI coordinates displayed in the screen capture below.
Place the cursor on IHA4 and click [#2].
Because you have selected the type ECOG-mid, the contacts are not be aligned on the skull or the cortex automatically. The contacts between the first and the last one are by default distributed uniformly on a straight line, which probably doesn't match their actual positions. Move slightly contacts IHA2 and IHA3 so that they appear in the middle of the artifacts.
Repeat the same steps with IHB: Set it as ECOG-mid, contact distance=10mm.
Place the cursor on IHB1 (the deepest contact on the middle strip) and click [#1].
Place the cursor on IHB4 and click [#2].
Move the IHB2 and IHB3 contacts on the slices to center them on the artifacts.
Repeat the same steps with IHC: Set it as ECOG-mid, contact distance=10mm.
Place the cursor on IHC1 (the deepest contact on the anterior strip) and click [#1].
Place the cursor on IHC4 and click [#2].
Move the IHC2 and IHC3 contacts on the slices to center them on the artifacts.
sEEG electrodes: TA, TB
In the iEEG tab, select electrode TA and edit the options:
Number of contacts: 10
Contact spacing: 10 mm
Contact length: 2.5 mm
Contact diameter: 1 mm
Electrode diameter: 0.9 mm
Electrode length: 120 mm
- The contacts actually have the same diameter as the electrode itself, but we use a slightly higher diameter for display, so that they render well in most 3D and 2D views.
- sEEG electrodes are defined with two points, the tip of the electrode (deepest contact) and a second point anywhere along the axis the electrode. One typical solution is to use the entry point in the skull as this second point, because the screws are always easy to identify. In this example, the electrodes are curved, and the entry point is not aligned with most of the contact, therefore we suggest to pick the last contact on the electrode instead. The intermediate contacts are placed along electrode, separated according to the "contact spacing" option, starting from the tip.
Place the cursor on TA1 (the deepest contact on the more mesial probe) and click [Set tip]. To jump directly to the coordinates of this point: click on the button Set current coordinates at the bottom of the MRI viewer, and report the MRI coordinates displayed in the screen capture below.
Place the cursor on TA10 (the last contact on this electrode) and click [Set skull entry].
This is obviously not the location of the screw in the skull, but as the electrode is bent, this is a better point to approximate the contact locations on a straight line.
The electrode is curved, therefore you need to move most of the intermediate contacts TA2-TA9 to more appropriate positions, at the center of the matching artifact. Unfortunately, Brainstorm does not handle well curved electrodes yet: if you move the contacts, the electrode remains represented as a straight line. This is a display issue only, which has no impact on any computation, but we hope to improve it at some point.
Repeat the same steps with TB: Set it as SEEG, and use the same configuration.
Place the cursor on TB1 (the deepest contact on the more mesial probe) and click [Set tip].
Place the cursor on TB10 (the last contact on this electrode) and click [Set skull entry].
Move the intermediate contacts TB2-TB9 to the centers of the artifacts.
When you are done with marking all the electrodes, click on [Save] in the MRI viewer.
Right-click on the channel file > Display sensors > Explore all the options available.
For help with the display options in the iEEG tab: Epileptogenicity tutorial.
You can add more anatomy elements to the figure with the button "Add a surface" at the top-right of the Surface tab. For more help: Display the anatomy.
Project on brain surface
You probably noticed that many the contacts of the ECoG grid appear under the surface of the cortex in these 3D figures. This is mostly due to the brain shift between the volumes we used to mark the contacts (post-implantation MRI) and the volume from which the cortex surface was reconstructed (pre-implantation MRI). One solution to compensate for this brain shift and produce more readable figures is to project the contacts on the pre-implantation surfaces.
In the iEEG tab, select the grid G, then use menu Contacts > Project on cortex.
You could also test what you obtain by projecting the contacts on the inner skull. It produces similar results, but with a different method, projecting the contacts possibily a bit further away from the brain. Depending on the quality of the surfaces, the shape of the brain, and the position of the contacts, one solution may produce weird results, try the other one in that case.
Alternative: Read BIDS electrodes.tsv
A BIDS dataset may contain information about the localization of the iEEG contacts in different systems of coordinates. These files are documented in the in BIDS specification. In this dataset, we can find the following files in the folder sample_ecog/sub-ecog01/ses-postimp/ieeg. Files
sub-ecog01_ses-postimp_space-IXI549Space_coordsystem.json: MNI space metadata
sub-ecog01_ses-postimp_space-IXI549Space_electrodes.tsv: XYZ coordinates, MNI space
sub-ecog01_ses-postimp_space-Other_coordsystem.json: Subject space metadata
sub-ecog01_ses-postimp_space-Other_electrodes.tsv: XYZ coordinates, subject space
The tag space- indicates how the coordinates should be interpreted (list of possible values):
IXI549Space: Refers to a MNI normalization computed by SPM12 (see Coordinate systems). You should be able to load these locations on any volume that was for which MNI coordinates are available, but with the approximations due to the volume-based normalization and possible errors due to the use of different types of normalization. When possible, always prefer importing coordinates in subject space.
Other: Refers to any non-standard space. The interpretation of the coordinates in the file electrodes.tsv requires the information available in the adjacent file coordsystem.json. Typically, this pair of files defines coordinates associated to a specific volume in the BIDS dataset.
For the subject space (space-Other), the contents of the json file is the following:
"iEEGCoordinateSystem": "Other", "iEEGCoordinateUnits": "mm", "iEEGCoordinateSystemDescription": "Scanner-based reference of the FreeSurfer T1.mgz", "iEEGCoordinateProcessingDescription": "SEEG contacts manually placed with Brainstorm on the post-implanation T1 MRI, which was resliced on the FreeSurfer T1.mgz", "IntendedFor": "derivatives/freesurfer/sub-ecog01_ses-preimp/mri/T1.mgz"
This means that the coordinates in the files sub-ecog01_ses-postimp_space-Other_electrodes.tsv refer to the volume T1.mgz available in the FreeSurfer segmentation. As this is the volume we used as the anatomical reference for the subject in this Brainstorm protocol, we should be able to import it. If the field IntendedFor of the json file was pointing at a different volume (e.g. sample_ecog/sub-ecog01/ses-postimp/anat/sub-ecog01_ses-postimp_T1w.nii.gz), we would not be able to import it in this subject and would need to use the MNI coordinates instead (sub-ecog01_ses-postimp_space-IXI549Space_electrodes.tsv).
To add the coordinates in subject space (don't do it if already marked the contacts manually):
Right-click on the channel file > Add EEG positions > Import from file
Select format "EEG: BIDS electrodes.tsv, subject space mm"
(or adjust depending on the tsv file you select and the information defined in the json file)
Select file sub-ecog01_ses-postimp_space-other_electrodes.tsv
Apply MRI transformation: Yes.
This will intepret the coordinates from the file electrodes.tsv as world coordinates in the reference volume of the subject (i.e. the first one that was imported: the FreeSurfer T1.mgz from the derivatives folder).
Note that the electrodes.tsv file does not include all the information we defined manually in the previous section (ECOG vs. ECOG-mid, contact diameter...), therefore for optimal display you might need to go through the configuration of each implanted device in the iEEG tab.
Display the recordings
ECoG/sEEG time series
Right-click on any "Link to raw file" > ECOG+SEEG > Display time series.
- For instructions on how to use this viewer, please refer to these tutorials:
- Typical display configuration includes:
Set the page duration to 20s or 30s: Record tab > Page settings.
Change the display mode to "columns": Record tab > Toolbar > First button.
Change the montage (bipolar/monopolar): Record tab > Drop-down menu.
- Set visualization filters: Filter tab.
- Disable the automatic scaling when changing page: Button [AS] in the time series figure.
- Adjust the amplitude scale: Right-click+move up/down, or buttons [^] and [v].
Set the resolution precisely: Right-click on the figure > Figure > Set axes resolution.
- Montage options include, for each group separately or all the channels together:
Bipolar 1: Difference of pairs of adjacent sensors (sparse), eg. A1-A2, A3-A4, A5-A6
Bipolar 2: Difference of pairs of adjacent sensors (exhaustive), eg. A1-A2, A2-A3, A3-A4
Local average reference: Average reference computed independently for each group
With the menus 2DLayout, 2D Electrodes and 3D Electrodes you can visualize the ECoG/sEEG values at the current time point. Move the time cursor and it will update the values represented on the contacts.
Interpolate on the anatomy
From the same ECOG+SEEG display menu, you can also project the recordings on the MRI or the cortex. You can edit the colormap for the displayed values like any other colormap.
MRI volume: All the voxels in the neighborhood of a contact are attributed the value associated with this contact. In the figure below, both "MIP: functional" and "MIP: anatomy" options are selected (maximum intensity projection along each axis = "glass brain" view).
Cortex surface: The values of the vertices are interpolated from the nearest SEEG contacts (the magnitude decreases with the distance to the contact).
We recommend you always start your data analysis with a spectral evalution of the recordings, it may help you identify bad channels. This is described in tutorials Power spectrum and EEG and epilepsy.
- In Process1, select the continuous file.
Run process Frequency > Power spectrum density (Welch): All file, Length=10s, ECOG,SEEG.
Double-click on the PSD files to display them, change to a log-scale for the X axis, zoom in. None of the channels seem to have noise levels that are higher or lower than the others, all the channels are possibly good.
- If the spectrum plots are too noisy and difficult to read, you can recompute them with lower frequency resolution. Decrease the duration of the estimator window, possibly down to 1s depending on the sampling frequency. This will estimate the PSD by averaging the power of more time windows with less data bins, the result will be smoother.
Import epochs of interest
At this point of the analysis, we are still looking at the original files, no data was copied to the database. The montages are saved in the Brainstorm preferences, the bad channels and new events are saved in the links of the database but not reported to the original files. If you delete your protocol at this point, you would only lose the event marking and bad channel selection.
We are now going to import a segment a recordings around each of the three seizures marked in this file (10s before and 20s after the onset). This will make real copies of the data in the database, so we can run additional processes on them.
- Double-click on the on the "Link to raw file" to open the time series figure (ECOG+SEEG).
In the Record tab: double-click on the event category seizure_begin and rename it seizure.
- Close the file viewer to save the modification to the events.
Right-click on Link to raw file > Import in database:
- Time window: All
- Split in time blocks: Disabled
Use events: Enabled, select seizure
Epoch time: [-10000, +20000] ms (imports -10s to 20s around the event "seizure")
- Remove DC offset: Disabled
- Resample: Disabled
Create a separate folder for each event type: Disabled
- At the end, you should have three new folders SZ1/SZ2/SZ3, the same name as the original .TRC files, but without the tag "raw" on top. These new folders contain copies of the SEEG recordings, if you delete these folders from the database explorer, you lose the recordings they contain.
The imported epochs are saved with a new timing: for the seizure onsets, the reference time t=0s is now the event "Onset", which has been removed from the list. You can still see the other marker Seizure, which should be slightly before 0s. For the baselines, the reference t=0 is the beginning of the segment.
As illustrated in the introduction tutorial Explore the anatomy, all the anatomical atlases available in the subject (e.g. DKT, Desikan-Killiany, Destrieux) are available in the MRI Viewer. Select the current atlas from the figure popup menu, and the anatomical label under the cursor is displayed at the top-right corner. You can overlay all the labels on the MRI with the menu Show atlas.
We can use these anatomical parcellations, and also the surface parcellations of the cortex available as scouts, in order to label automatically all the ECOG/SEEG contacts.
Right-click on the channel file > iEEG atlas labels > Select all the available options: coordinates in various coordinate systems, volume parcellations, surface parcellations.
More options and details in the tutorial SEEG epileptogenicity maps.