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FEM tensors estimation with BrainSuite

Authors: Takfarinas Medani, Francois Tadel, Anand Joshi and Richard Leahy

[TUTORIAL UNDER WRITING: NOT READY FOR PUBLIC USE]

In this tutorial, we describe the process of the estimation of the realistic conductivity tensors for the brain tissues using the BrainSuite software. The main purpose is to generate the conductivity tensor for the FEM computation as introduced on this page.

https://neuroimage.usc.edu/brainstorm/Tutorials/SegBrainSuite?highlight=%28anand%29

The realistic tensors are estimated from the Diffusion-Weighted Images (DWI). For this purpose, Brainstorm calls internally the BrainSuite Diffusion Pipeline to compute the diffusion tensors on each brain voxel. Afterward, the Effective Medium Approach is applied to convert the diffusion tensors to the conductivity tensors. The following section shows the users how to do it from the graphical interface.

Only the NIfTI is supported. All the diffusion data, including the DWI file and direction and the value of the gradient files, respectively the *.nii, the *.bval and the *.bvec are required. Ideally, these files should have the same name and saved in the same folder.

Contents

Requirement
Brainsuite Installation
Dataset
Realistic condctivity tensors
Artificial/simulated conductivity tensors
References

Requirement

You have already followed all the introduction tutorials
You have a working copy of Brainstorm installed on your computer

Brainsuite Installation

Download the latest version of BrainSuite from http://www.brainsuite.org/download.
Install it on your computer by following the instructions in BrainSuite's quick start installation guide.
Note that you will be using BrainSuite Diffusion Pipeline(BDP), so you need to install a compatible MATLAB Compiler Runtime(last version).
Start BrainSuite to check if the installation (It's not required to open BrainSuite to run this tutorial).
The BrainSuite installation folder should be informed in the Brainstorm preferences

Dataset

In this tutorial, we use the Brainsuite dataset example available on the BrainSuite tutorial webpage. User can also download directly these data from these links: MRI T1w MRI DWI

The first file contains the T1 MRI data, with the name '2523412.nii.gz'. as in this figure,

The second file is the DWI and should contain at least three files

Where the *.bval is a text file that contains the value of the gradient, and the *.bvec is also a text file that contains the orientation of the gradient. The nii.gz file is the NifTi file of the DWI where the images are stored.

Realistic condctivity tensors

Load the T1 MRI data to brainstorm

First, you need to create a new subject in your protocol, let call it the 'BrainSuiteSubject'. Then import the T1 MRI of the subject and set the fiducials points as explained in the previous tutorial.

The T1 MRI is required since all the BDP uses the T1 space for its computation. Furthermore, this is required since it will be used to align the tensors to the FEM mesh later.

Diffusion tensor generation (DTI) from DWI

In this step, Brainstorm calls the Brainsuite internally, and the diffusion tensors are computed.

Right-click on the subject and then select the item "Convert DWI to DTI".

Then follow the popup windows by selecting the DWI,

If the bval, and bval files are in the same folder, Brainstorm will detect them automatically, otherwise, the user will be asked to browse the files one by one (as it's the case in this tutorial).

Brainstorm calls internally the BrainSuite process, and compute the diffusion tensors.

At the end of this process, a new node will appear in the Brainstorm database with the name 'DTI-EIT'. This name refers to, DTI: diffusion tensors images, and EIG for eigenvalue, since the eigenvalues and eigenvectors are computed at voxel and stored in Brainstorm database.

If you check the structure of the file DTI-EIG, by right click -> File and then 'Display file contents', the following figure is displayed.

The size of the matrix is 128x256x256x12, where the first 3 values are the same as the size of the T1 MRI and 12 corresponds to the 3 eigenvectors components (9) and eigenvalues (3)

Conductivity tensor generation from DTI

The Effective Medium Approach is applied to convert the diffusion tensors to the conductivity tensors.

FEM mesh head model

This step requires the FEM mesh of the head model. You can generate the FEM head model from the MRI data as explained on this page.

For the following, we used the SimNibs FEM mesh generation. The following figure shows the FEM mesh obtained with the SimNibs method using the T1 MRI.

Note that this mesh is obtained only from the T1, the use of the T2 is highly recommended if it's available, as recommended in the FEM mesh tutorial.

Computation of FEM mesh tensors

Once the FEM mesh and the DTI tensors are available in the Brainstorm database, the next step for the FEM tensors can be performed by the following:

- Right-click on the FEM mesh - Compute FEM tensors

Brainstorm checks the available tissues in the FEM head model and displays the following panel

This panel lists the tissues available in the FEM head model and assigns a default value of the conductivity for each compartment. Users can change these values to their own if needed.

DTI values can be used to generate conductivity tensors for the white matter (and in some cases for the grey matter). Please, note that the DWI can be used only for the brain tissues and not for the outers compartments (skull and skin)

In this tutorial (and in most cases) we select the white matter. Select the WM anisotropy and keep all the other tissues as isotropic, then these additional options appear asking for the method to use.

The available methods are:

- Effective Medium approach (EMA)

- Effective Medium approach with volume constraints (EMA + VC)

- Simulated or the artificial anisotropy

Only the two first methods require the DTI. More information about these methods can be found on these references [ref1][ref2] and in our main paper [link]

In this tutorial, we use the method "EMA + VC", where the final tensors are constrained to fits the volume of the equivalent isotropic tensor volume.

Visulation of FEM mesh tensors

Once the FEM tensors are successfully computed, they are stored in the FEM head node. By right-clicking on the FEM head, new menu items are added that gives the possibilities to display the FEM tensors either as ellipsoids or as vectors in the direction of the main eigenvector.

The tensors can be displayed either on the FEM mesh or overlaid on the MRI. The following figures show an example of the obtained tensors displayed on the white matter.

On the left, the tensors as a line on the direction of the main eigenvector. On the right, the tensors displayed as ellipsoids. The orientation of the tensor is color-coded as follows: red for right-left, green for anterior-posterior, and blue for superior-inferior.

Note that the quality of the tensors depends on the DWI data and the number of acquisition direction.

Users can also display the tensors on specific tissues, for example on the white matter (left figure) or overlay on the MRI (right figure).

Recommendation

In the case where the user wants to use generate isotropic tensors, then the DTI is not required. In the case where more than one FEM head model is in the database, the highlighted one in the green color will be used.

Advanced

Artificial/simulated conductivity tensors

In the case where the DWI is not available, or in the case where the users desire to evaluate the effect of the conductivity change on the model, the artificial conductivity can be used.

Two approaches are integrated within Brainstorm. Either the

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-Describe brainsuite here : here we will describe the process of the brain tissues anisotrpy estimation and the different functions that brainstorm offers.
+In this tutorial, we describe the process of the estimation of the realistic conductivity tensors for the brain tissues using the [[http://brainsuite.org/|BrainSuite software]].  The main purpose is to generate the conductivity tensor for the FEM computation as introduced on [[https://neuroimage.usc.edu/brainstorm/Tutorials/Duneuro|this page]].
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-This tutorial explains how to use Brainsuite to estimate the anisotropy of the brain tissues.
+[[https://neuroimage.usc.edu/brainstorm/Tutorials/SegBrainSuite?highlight=(Anand)|https://neuroimage.usc.edu/brainstorm/Tutorials/SegBrainSuite?highlight=%28anand%29]]
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-refer to this page
+The realistic tensors are estimated from the Diffusion-Weighted Images (DWI). For this purpose, Brainstorm calls internally the BrainSuite Diffusion Pipeline to compute the diffusion tensors on each brain voxel. Afterward, the Effective Medium Approach is applied to convert the diffusion tensors to the conductivity tensors. The following section shows the users how to do it from the graphical interface.
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-[[https://neuroimage.usc.edu/brainstorm/Tutorials/SegBrainSuite?highlight=(anand)|https://neuroimage.usc.edu/brainstorm/Tutorials/SegBrainSuite?highlight=%28anand%29]]

The realistic tensors are estimated from the Diffusion Weighted Images (DWI). For this purpose, Brainstorm calls internally the BrainSuite Diffusion Pipline to compute the diffusion tensors on each brain voxel. Afterwards, the Effective Medium Appeach is applied to convert the diffusion tensors to the conductivity tensors. The following section shows to the users how to do it from the graphical intefrace.

Only the NIfTI are supported. All the diffusion data, inclusing the DWI file and direction and the value of the gradient files , respectively the the *.nii, the *.bval and the *.bvec are required. Ideally these files should have the same name and saved in the same folder.
+Only the NIfTI is supported. All the diffusion data, including the DWI file and direction and the value of the gradient files, respectively the *.nii, the *.bval and the *.bvec are required. Ideally, these files should have the same name and saved in the same folder.
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-{{https://user-images.githubusercontent.com/6920058/81406567-1c785400-913a-11ea-9048-28c7459af7da.png|image}}
+{{attachment:brainsuiteInstalation.JPG||height="250",width="650"}}
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+----
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-In this tutorial we use the Brainsuite dataset example available on the Brainsuite tutorial webpag
+In this tutorial, we use the Brainsuite dataset example available on the BrainSuite  [[http://brainsuite.org/tutorials/|tutorial webpage]]. User can also download directly these data from these links: [[http://brainsuite.org/WebTutorialData/BrainSuiteTutorialSVReg_Sept16.zip|MRI T1w]] [[http://brainsuite.org/WebTutorialData/DWI_Feb15.zip|MRI DWI]]
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-http://brainsuite.org/tutorials/. The T1w of the subject can be download from this link  [[http://brainsuite.org/WebTutorialData/BrainSuiteTutorialSVReg_Sept16.zip|BrainSuiteTutorialSVReg_Sept16.zip]]. The DWI from this link  [[http://brainsuite.org/WebTutorialData/DWI_Feb15.zip|DWI_Feb15.zip]]
+The first file contains the T1 MRI data, with the name '2523412.nii.gz'. as in this figure,
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-The first link contains the T1 MRI, with the name '2523412.nii.gz'
+{{attachment:vierMriFolder.JPG||height="130",width="580"}}
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-Where the *.bval is a text file that contains the value of the gradient, and the *.bvec is also a text file that contaons the orientation of the gradient. The nii.gz file is the NifTi file of the DWI where the images are stored.
+{{attachment:vierDWIFolder.JPG||height="150",width="580"}}

Where the *.bval is a text file that contains the value of the gradient, and the *.bvec is also a text file that contains the orientation of the gradient. The nii.gz file is the NifTi file of the DWI where the images are stored.
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+Line 50:
-First you need to create a new subject in your protocole, let call it the 'BrainSuiteSubject'. Then import the T1 MRI of the subject and set the fidicials points as explained in the previous tutorial.
+=== Load the T1 MRI data to brainstorm ===
First, you need to create a new subject in your protocol, let call it the 'BrainSuiteSubject'. Then import the T1 MRI of the subject and set the fiducials points as explained in the [[https://neuroimage.usc.edu/brainstorm/Tutorials/ImportAnatomy|previous tutorial]].
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+Line 53:
+The T1 MRI is required since all the BDP uses the T1 space for its computation. Furthermore, this is required since it will be used to align the tensors to the FEM mesh later.
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+Line 55:
+{{attachment:mri.JPG||height="100",width="250"}}
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+Line 57:
-=== Diffusion tensor generation from DWI ===
Right click on the subject and then select the item "Convert DWI to DTI".
+=== Diffusion tensor generation (DTI) from DWI ===
In this step, Brainstorm calls the Brainsuite internally, and the diffusion tensors are computed.
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+Line 60:
-Then follow the popup windows by selecting the DWI, bval and bval. If these files are in the same folder, Brainstorm will detect them automatiquely, otherwise user will be asked to browse the files one by one (as it's  the case in this tutorial).
+Right-click on the subject and then select the item "Convert DWI to DTI".
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+Line 62:
-In this step, Brainstorm calls the Brainsuite internally, and the the diffusion tensors are computed. At the end of this process, a new node will appeare in the Brainstom database with the name 'DTI-EIT'. This name refers to, DTI: diffusion tensors images, and EIG for eigen value, since the eigenvalues and eigenvectors are computed at voxel and stored in Brainstorm database.
+{{attachment:dwi2dti.jpg||height="300",width="550"}}
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+Line 64:
-You have already generated the FEM mesh as explained here (link to the FEM mesh tutorial)
+Then follow the popup windows by selecting the DWI,
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-The FEM head model to use for tensors should be selected and highlighted with the green color (double click on the FEM mesh node to select it)
+{{attachment:importDWI.JPG||height="280",width="650"}}
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+Line 68:
-When this is done, then right-click on the subject > Convert DWI to DTI,
+If the bval, and bval files are in the same folder, Brainstorm will detect them automatically, otherwise, the user will be asked to browse the files one by one (as it's the case in this tutorial).
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+Line 70:
-Brainstorm will load the avialbale tissue in the FEM head model and the following windows appears.
+Brainstorm calls internally the BrainSuite process, and compute the diffusion tensors.
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-Select the WM anisotropy and kee all the oher tissues as isotropic.
+At the end of this process, a new node will appear in the Brainstorm database with the name 'DTI-EIT'. This name refers to, DTI: diffusion tensors images, and EIG for eigenvalue, since the eigenvalues and eigenvectors are computed at voxel and stored in Brainstorm database.
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+Line 74:
-The process of conversion from DWI to Conductivity tensors use the EMA, furthermore, brainstorm propose the option to use the adaptative EMA with the volume constraint option [ref]. In this example we select the EMA with the VC.
+{{attachment:mriAndDTI-EIG.JPG||height="100",width="230"}}
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+Line 76:
-The process will take around 10 min, and then the FEM tensors are computed and stored in the FEM strucutre. [explain how it is organised and how to use it outside brainstorm ]
+If you check the structure of the file DTI-EIG, by right click -> File and then 'Display file contents', the following figure is displayed.
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+Line 78:
-=== Display the tensors ===
+{{attachment:EIG-hardDisc.JPG||height="200",width="450"}}

The size of the matrix is 128x256x256x12, where the first 3 values are the same as the size of the T1 MRI and 12 corresponds to the 3 eigenvectors components (9) and eigenvalues (3)

=== Conductivity tensor generation from DTI ===
The Effective Medium Approach is applied to convert the diffusion tensors to the conductivity tensors.

==== FEM mesh head model ====
This step requires the FEM mesh of the head model. You can generate the FEM head model from the MRI data as explained on [[https://neuroimage.usc.edu/brainstorm/Tutorials/FemMesh|this page]].

For the following, we used the SimNibs FEM mesh generation. The following figure shows the FEM mesh obtained with the SimNibs method using the T1 MRI.

{{attachment:Mri&femMeshView.JPG|Mri&femMeshView.JPG|height="300",width="260"}} {{attachment:femMeshView.JPG||height="300",width="280"}}

Note that this mesh is obtained only from the T1, the use of the T2 is highly recommended if it's available, as recommended in the [[https://neuroimage.usc.edu/brainstorm/Tutorials/FemMesh|FEM mesh tutorial]].

==== Computation of FEM mesh tensors ====
Once the FEM mesh and the DTI tensors are available in the Brainstorm database, the next step for the FEM tensors can be performed by the following:

- Right-click on the FEM mesh - Compute FEM tensors

{{attachment:menuGenerateFemTensors.png||height="280",width="250"}}

Brainstorm checks the available tissues in the FEM head model and displays the following panel

{{attachment:FEMConductivitiesIsoPanel.JPG||height="220",width="250"}}

This panel lists the tissues available in the FEM head model and assigns a default value of the conductivity for each compartment. Users can change these values to their own if needed.

DTI values can be used to generate conductivity tensors for the white matter (and in some cases for the grey matter). Please, note that the DWI can be used only for the brain tissues and not for the outers compartments (skull and skin)

In this tutorial (and in most cases) we select the white matter. Select the WM anisotropy and keep all the other tissues as isotropic, then these additional options appear asking for the method to use.

{{attachment:FEMConductivitiesAnisoPanel.JPG||height="300",width="250"}}

The available methods are:

- Effective Medium approach (EMA)

- Effective Medium approach with volume constraints  (EMA + VC)

- Simulated or the artificial anisotropy

Only the two first methods require the DTI. More information about these methods can be found on these references [ref1][ref2] and in our main paper [link]

In this tutorial, we use the method "EMA + VC", where the final tensors are constrained to fits the volume of the equivalent isotropic tensor volume.

==== Visulation of FEM mesh tensors ====
Once the FEM tensors are successfully computed, they are stored in the FEM head node. By right-clicking on the FEM head, new menu items are added that gives the possibilities to display the FEM tensors either as ellipsoids or as vectors in the direction of the main eigenvector.

{{attachment:menuDisplayTensors.jpg||height="300",width="250"}}

The tensors can be displayed either on the FEM mesh or overlaid on the MRI. The following figures show an example of the obtained tensors displayed on the white matter.

{{attachment:meshViewTensorsLines.JPG||height="300",width="350"}} {{attachment:meshViewTensorsTensorsTops.JPG||height="300",width="350"}}

On the left, the tensors as a line on the direction of the main eigenvector. On the right, the tensors displayed as ellipsoids. The orientation of the tensor is color-coded as follows: red for right-left, green for anterior-posterior, and blue for superior-inferior.

Note that the quality of the tensors depends on the DWI data and the number of acquisition direction.

Users can also display the tensors on specific tissues, for example on the white matter (left figure) or overlay on the MRI (right figure).

{{attachment:meshViewTensorsLinesWM2.JPG||height="300",width="350"}} {{attachment:tensorsOnMri.JPG||height="300",width="300"}}

==== Recommendation ====
In the case where the user wants to use generate isotropic tensors, then the DTI is not required. In the case where more than one FEM head model is in the database, the highlighted one in the green color will be used.

<<TAG(Advanced)>>
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+Line 148:
-In the case where the DWI is not available, or the users desire to evaluates the effect of the conductivity change on the model, the artificial conductivity can be use.
+In the case where the DWI is not available, or in the case where the users desire to evaluate the effect of the conductivity change on the model, the artificial conductivity can be used.

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