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[TUTORIAL UNDER CONSTRUCTION: NOT READY FOR PUBLIC USE]

FEM tensors estimation with BrainSuite

Authors: Takfarinas Medani, Francois Tadel, Anand Joshi and Richard Leahy

In this tutorial, we describe the estimation of realistic conductivity tensors of living brain tissues using the BrainSuite software. These results are used in FEM forward modeling, as described in the tutorials: FEM with DUNEuro and FEM median nerve example.

The realistic tensors are estimated from the Diffusion-Weighted Images (DWI): Brainstorm calls the BrainSuite software to compute the diffusion tensors on each brain MRI voxel (DTI), then Effective Medium Approach (EMA) is applied to estimate the conductivity tensors for each element of a tetrahedral FEM mesh. This is particularly interesting for the modeling the anisotropy of the white matter.

BrainSuite is also used for other purposes in Brainstorm, particularly the T1 MRI segmentation, as documented in this tutorial: MRI segmentation: BrainSuite.

Contents

Download and installation
Import the anatomy
FEM mesh
FEM conductivity tensors
Simulated conductivity tensor
Troubleshooting
References

Download and installation

Requirements

You have already followed all the introduction tutorials.
You have a working copy of Brainstorm installed on your computer.
For the DWI data, only the NIfTI files (.nii) are supported.

Install Brainsuite

Download the latest version of BrainSuite from http://forums.brainsuite.org/download/.
Install it on your computer by following the instructions in BrainSuite's quick start installation guide.
You will be using BrainSuite Diffusion Pipeline (BDP), so you need to install a compatible MATLAB Runtime (2019b for BrainSuite 21a).
In Brainstorm, menu File > Edit preferences > Enter the BrainSuite installation folder:

Download the dataset

Download the files: MRI T1w and MRI DWI (from the BrainSuite diffusion tutorial).
Unzip it outside of any of the Brainstorm folders (program folder or database folder).
Start Brainstorm (Matlab scripts or stand-alone version)
Select the menu File > Create new protocol. Name it "TutorialTensors" and select:
- No, use individual anatomy
- No, use one channel file per condition

Import the anatomy

T1 MRI

Switch to the "anatomical data" view, the left button in the toolbar above the database explorer.
Right-click on the TutorialFem folder > New subject > Subject01
- Keep the default options you set for the protocol.
Right-click on the subject node > Import MRI:
- Set the file format: All MRI files (subject space)
- Select the T1 file: BrainSuiteTutorialSVReg/2523412.nii.gz
Click on the link "Click here to compute MNI normalization": option "maff8". This estimates an affine transformation to the MNI space and sets default positions for the anatomical fiducials. The NAS/LPA/RPA fiducials are needed for defining the Brainstorm subject coordinate system, in which the surfaces and FEM meshes are stored.

Diffusion imaging

This computes the This requires BrainSuite to be installed on your computer, with the bdp program available in the system path.

Right-click on Subject01 > Convert DWI to DTI
Select the DWI file: DWI/2523412.dwi.nii.gz
The associated text files *.bvec (orientation of the gradient) and *.bval (value of the gradient) must be in the same folder, with the same file name. Theses files are created from for the DWI acquisition. If you don't have them, ask the person who programmed your DWI sequence and get the files that are specific to your use case.
The process can take up to 30min. At the end, a new file DTI-EIG appears in the database (DTI=diffusion tensors images, EIG=eigenvalue). This file contains 12 volumes, ie. 12 values for each voxel. From 1 to 9: components of the three eigenvectors; from 10 to 12: the values of their norm to the eigenvalue.

FEM mesh

The FEM approach requires a segmentation of the head volume in different tissues, represented as hexahedral or tetrahedral 3D meshes. The methods available within Brainstorm are listed in the tutorial FEM mesh generation.

Here we illustrate only the use of Brain2mesh: this is not the most accurate solution for MRI segmentation but it is probably the fastest solution to obtain a tetrahedral mesh of the head with 5 tissues (gray matter, white matter, CSF, skull, skin). For more accurate results, we recommend using SimNIBS with T1+T2, as illustrated in the tutorial FEM median nerve example.

Right-click on the T1 MRI > MRI segmentation > Generate FEM mesh > Brain2mesh.
After less than 15 minutes, you will obtain a new FEM mesh in the database.

FEM conductivity tensors

Once the FEM mesh and the DTI tensors are available in the Brainstorm database, the next step is to compute the conductivity tensor for each of the FEM mesh element.

Right-click on the FEM mesh > Compute FEM tensors.
Brainstorm checks the available tissues in the FEM mesh and assigns a default isotropic conductivity value of the conductivity for each compartment. Users can change these values to their own if needed.
When selecting "Anisotropic" for a tissue, the DTI values are used to generate conductivity tensors: this is used mostly for the white matter (and in some cases for the grey matter). Note that DWI can be used only for the brain tissues and not for the outers compartments (skull and skin). The methods available are:
- EMA: Effective Medium Approach: See (Tuch 2001).
- EMA + VC: EMA with volume constraints: The final tensors are constrained to fit the volume of the equivalent isotropic tensor volume.
- Simulated: Artificial anisotropy (DTI is not required)
For this example, select: WM:Anisotropic and Isotropic for the other tissues, EMA+VA.
The FEM tensors are saved in the the FEM mesh file, in the field Tensors.

Visualization

Once the FEM tensors are successfully computed, they are stored in the FEM mesh file. By right-clicking on the FEM head, new menu items are added that gives the possibilities to display the FEM tensors either as ellipsoids or as vectors in the direction of the main eigenvector.

The tensors can be displayed either on the FEM mesh or overlaid on the MRI. The following figures show an example of the obtained tensors displayed on the white matter.

On the left, the tensors as a line on the direction of the main eigenvector. On the right, the tensors displayed as ellipsoids. The orientation of the tensor is color-coded as follows: red for right-left, green for anterior-posterior, and blue for superior-inferior.

Note that the quality of the tensors depends on the DWI data and the number of acquisition direction.

Users can also display the tensors on specific tissues, for example on the white matter (left figure) or overlay on the MRI (right figure).

Recommendation

In the case where the user wants to use generate isotropic tensors, then the DTI is not required. For that case, keep all the options to 'isotropic', the recommended display is the 'Ellipsoids', and the final shape will be a sphere (isotropic direction).

Advanced

Simulated conductivity tensor

In the case where the DWI is not available, or in the case where the users desire to evaluate the effect of the conductivity change on the head model, the artificial conductivity can be used.

Users can reach this option by following this tutorial and select the third method in this panel.

Two approaches are integrated within Brainstorm. Either Wang's constraint or the volume's constraint (Wolters). The common feature between these methods is the ratio between the transversal and longitudinal conductivity ratio.

A common example is the skull anisotropy simulation, where the longitudinal conductivity can be higher than the transversal conductivity, the ratio can vary from 2 to 10 [ref]. In this tutorial, we keep all the tissue as isotropic, except the skull, we use a ratio of 0.1 and select the volume constraint. The following figures show the results of this example.

"eigenvalues parallel (longitudinal) and perpendicular (transverse) to the fiber directions" for 1:10 anisotropy (transverse:longitudinal)

Troubleshooting

To be completed soon and linked to BrainSuite website

References

===TODO===

Check the error in the simnibs mesh in X direction and overlay on mri check the error with the brain2mesh Correct the ratio from integer to float check the meaning of transversal/longitidunal in the code add an interactive way yo change the size of the tensor.. important correct the name of the simulated method, correct the EMC and remove the VC and change the coefficcient

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-= Estimation of brain's tissues anisortopy using Brainsuite Diffusion Pipline =
+'''[TUTORIAL UNDER CONSTRUCTION: NOT READY FOR PUBLIC USE]'''
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-Describe brainsuite here : here we will describe the process of the brain tissues anisotrpy estimation and the different functions that brainstorm offers.
+----

= FEM tensors estimation with BrainSuite =
''Authors: [[https://neuroimage.usc.edu/brainstorm/AboutUs/tmedani|Takfarinas Medani]], Francois Tadel, Anand Joshi and Richard Leahy''

In this tutorial, we describe the estimation of realistic conductivity tensors of living brain tissues using the [[http://brainsuite.org/|BrainSuite software]].   These results are used in FEM forward modeling, as described in the tutorials: [[https://neuroimage.usc.edu/brainstorm/Tutorials/Duneuro#DUNEuro_options:_Advanced|FEM with DUNEuro]] and [[https://neuroimage.usc.edu/brainstorm/Tutorials/FemMedianNerve#FEM_tensors|FEM median nerve example]].

The realistic tensors are estimated from the Diffusion-Weighted Images (DWI): Brainstorm calls the BrainSuite software to compute the diffusion tensors on each brain MRI voxel (DTI), then Effective Medium Approach (EMA) is applied to estimate the conductivity tensors for each element of a tetrahedral FEM mesh. This is particularly interesting for the modeling the anisotropy of the white  matter.

BrainSuite is also used for other purposes in Brainstorm, particularly the T1 MRI segmentation, as  documented in this tutorial: [[Tutorials/SegBrainSuite|MRI segmentation: BrainSuite]].

<<TableOfContents(2,2)>>

== Download and installation ==
==== Requirements ====
 * You have  already  followed all the  introduction tutorials.
 * You have a working copy of   Brainstorm  installed on your computer.
 * For the DWI data, only the NIfTI files (.nii) are supported.

==== Install Brainsuite ====
 1. Download the latest version of BrainSuite from http://forums.brainsuite.org/download/.

 1. Install it on your computer by following the instructions in [[http://brainsuite.bmap.ucla.edu/quickstart/installation/|BrainSuite's quick start installation guide]].

 1. You will be using BrainSuite Diffusion Pipeline (BDP), so you need to install a compatible [[https://www.mathworks.com/products/compiler/matlab-runtime.html|MATLAB Runtime]] (2019b for BrainSuite 21a).

 1. In Brainstorm, menu File > Edit preferences > Enter the BrainSuite installation folder:<<BR>><<BR>> {{attachment:brainsuiteInstall.gif}}

==== Download the dataset ====
 * Download the files: [[http://brainsuite.org/WebTutorialData/BrainSuiteTutorialSVReg_Sept16.zip|MRI T1w]] and [[http://brainsuite.org/WebTutorialData/DWI_Feb15.zip|MRI DWI]] (from the [[http://brainsuite.org/tutorials/dtiexercise/|BrainSuite diffusion tutorial]]).

 * Unzip it outside of any of the Brainstorm folders (program   folder or database folder).
 * Start Brainstorm (Matlab scripts or stand-alone version)
 * Select the menu File > Create new protocol. Name it "'''TutorialTensors'''" and select:
  * No, use individual anatomy
  * No, use one channel file per condition

== Import the anatomy ==
=== T1 MRI ===
 * Switch to the "anatomical data" view, the left button in the toolbar above the database explorer.
 * Right-click on the TutorialFem folder > New subject > '''Subject01'''
  * Keep the default options you set for the protocol.

 * Right-click on the subject node > '''Import MRI''':
  * Set the file format: '''All MRI files (subject space)'''

  * Select the T1 file: BrainSuiteTutorialSVReg/'''2523412.nii.gz'''

 * Click on the link "'''Click here to compute MNI normalization'''": option "'''maff8'''". This estimates an affine transformation to the [[https://neuroimage.usc.edu/brainstorm/CoordinateSystems#MNI_coordinates|MNI space]] and sets default positions for the anatomical fiducials. The NAS/LPA/RPA fiducials are needed for defining the Brainstorm [[CoordinateSystems|subject coordinate system]], in which the surfaces and FEM meshes are stored. <<BR>><<BR>> {{attachment:importT1.gif}}

=== Diffusion imaging ===
This computes the  This requires BrainSuite to be installed on your computer, with the bdp program available in the system path.

 * Right-click on Subject01''' '''> '''Convert DWI to DTI'''

 * Select the DWI file: DWI/'''2523412.dwi.nii.gz'''

 * The associated text files '''*.bvec''' (orientation of the gradient) and '''*.bval''' (value of the gradient) must be in the same folder, with the same file name. Theses files are created from for the DWI acquisition. If you don't have  them, ask the person who programmed your DWI sequence and get the files  that are specific to your use case.

 * The process can take up to 30min. At the end, a new file '''DTI-EIG'''  appears in the database (DTI=diffusion tensors images, EIG=eigenvalue). This file contains 12 volumes, ie. 12 values  for each voxel. From 1 to 9: components of the three eigenvectors; from  10 to 12:  the values of their norm to the eigenvalue.  <<BR>><<BR>> {{attachment:importDTI.gif}}

== FEM mesh ==
The FEM approach requires a segmentation of the head volume in different  tissues, represented as hexahedral or tetrahedral 3D meshes. The  methods available within Brainstorm are listed in the tutorial [[https://neuroimage.usc.edu/brainstorm/Tutorials/FemMesh|FEM mesh generation]].

Here we illustrate only the use of '''Brain2mesh''': this is not the most accurate solution for MRI segmentation but it is probably the fastest solution to obtain a tetrahedral mesh of the head with 5 tissues (gray matter, white matter, CSF, skull, skin). For more accurate results, we recommend using '''SimNIBS with T1+T2''', as illustrated in the tutorial [[https://neuroimage.usc.edu/brainstorm/Tutorials/FemMedianNerve#FEM_mesh_with_SimNIBS|FEM median nerve example]].

 * Right-click on the T1 MRI > MRI segmentation > '''Generate FEM mesh''' > '''Brain2mesh'''.<<BR>><<BR>> {{attachment:femMesh1.gif}}
 * After less than 15 minutes, you will obtain a new FEM mesh in the database.<<BR>><<BR>> {{attachment:femMesh2.gif}}

== FEM conductivity tensors ==
Once the FEM mesh and the DTI tensors are available in the Brainstorm database, the next step is to compute the conductivity tensor for each of the FEM mesh element.

 * Right-click on the FEM mesh > '''Compute FEM tensors'''.
 * Brainstorm checks the available tissues in the FEM mesh and assigns a default '''isotropic conductivity''' value of the conductivity for each compartment. Users can change these values to their own if needed.
 * When selecting "'''Anisotropic'''" for a tissue, the DTI values are used to generate conductivity tensors: this is used mostly for the '''white matter''' (and in some cases for the grey matter). Note that DWI can be used only for the brain tissues and not for the outers compartments (skull and skin). The methods available are:
  * '''EMA''': Effective Medium Approach: See [[http://www.pnas.org/content/98/20/11697|(Tuch 2001)]].
  * '''EMA + VC''': EMA with volume constraints: The final tensors are constrained to fit the volume of the equivalent isotropic tensor volume.
  * '''Simulated''': Artificial anisotropy (DTI is not required)
 * For this example, select: WM:Anisotropic and Isotropic for the other tissues, EMA+VA.<<BR>><<BR>>{{attachment:computeTensors.gif}}
 * The FEM tensors are saved in the the FEM mesh file, in the field Tensors.<<BR>><<BR>>{{attachment:femContents.gif}}

Visualization

Once the FEM tensors are successfully computed, they are stored in the FEM mesh file. By right-clicking on the FEM head, new menu items are added that gives the possibilities to display the FEM tensors either as ellipsoids or as vectors in the direction of the main eigenvector.

{{attachment:menuDisplayTensors.jpg||width="250",height="300"}}

The tensors can be displayed either on the FEM mesh or overlaid on the MRI. The following figures show an example of the obtained tensors displayed on the white matter.

{{attachment:meshViewTensorsLines.JPG||width="350",height="300"}} {{attachment:meshViewTensorsTensorsTops.JPG||width="350",height="300"}}

On the left, the tensors as a line on the direction of the main eigenvector. On the right, the tensors displayed as ellipsoids. The orientation of the tensor is color-coded as follows: red for right-left, green for anterior-posterior, and blue for superior-inferior.

Note that the quality of the tensors depends on the DWI data and the number of acquisition direction.

Users can also display the tensors on specific tissues, for example on the white matter (left figure) or overlay on the MRI (right figure).

{{attachment:meshViewTensorsLinesWM2.JPG||width="350",height="300"}} {{attachment:tensorsOnMri.JPG||width="300",height="300"}}

==== Recommendation ====
In the case where the user wants to use generate isotropic tensors, then the DTI is not required. For that case, keep all the options to 'isotropic', the recommended display is the 'Ellipsoids', and the final shape will be a sphere (isotropic direction).

<<TAG(Advanced)>>

== Simulated conductivity tensor ==
In the case where the DWI is not available, or in the case where the users desire to evaluate the effect of the conductivity change on the head model, the artificial conductivity can be used.

Users can reach this option by following this tutorial and select the third method in this panel. {{attachment:artificialTensors.JPG||width="280",height="450"}}

Two approaches are integrated within Brainstorm. Either Wang's constraint or the volume's constraint (Wolters). The common feature between these methods is the ratio between the transversal and longitudinal conductivity ratio.

A common example is the skull anisotropy simulation, where the longitudinal conductivity can be higher than the transversal conductivity, the ratio can vary from 2 to 10 [ref]. In this tutorial, we keep all the tissue as isotropic, except the skull, we use a ratio of 0.1 and select the volume constraint. The following figures show the results of this example.

"eigenvalues parallel (longitudinal) and perpendicular (transverse) to the fiber directions" for 1:10 anisotropy (transverse:longitudinal)

{{attachment:skullAniso.JPG||width="300",height="250"}} {{attachment:skullAniso2.JPG||width="300",height="250"}}

== Troubleshooting ==
To be completed soon and linked to BrainSuite website

== References ==
===TODO===

Check the error in the simnibs mesh in X direction and overlay on mri check the error with the brain2mesh  Correct the ratio from integer to float  check the meaning of transversal/longitidunal in the code add an interactive way yo change the size of the tensor.. important   correct the name of the simulated method,   correct the EMC and remove the VC and change the coefficcient

Software

Users

Development